GALK2 Stable Knockout HEK293T Cell Line

CAT.NOUNITPRICE
T61541x106 cells / 1.0 ml
$0.00

Specifications


DescriptionThe Galactokinase 2 (GALK2) is an efficient kinase of N-acetylgalactosamine in primary pathway for galactose metabolism the Leloir pathway that also acts as a galactokinase when galactose concentrations are high.
GALK2 Stable Knockout cell line was generated through the knockout of the GALK2 gene in HEK293T cells using the CRISPR-Cas 9 system. Cells were transiently transfected with an expression vector containing a GFP tag and a Guide RNA sequence that targeted GALK2. Clones were initially chosen through strong GFP expression and knockout of GALK2 confirmed via Western blot analysis, sequencing and GALK2 enzymatic activity. In combination with the GALE GALK2 Stable Knockout cell line (T6152), this line may prove useful in the study of O-linked glycosylation and galactose metabolism.
SKUT6154
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemKidney
Donor AgeEmbryo
Growth PropertiesAdherent
Cell MorphologyEpithelial
Seeding Density40,000 - 50,000 cells/cm2
Population Doubling Time33 - 43 hours
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetEnzymes
Cell TypeDrug Discovery Cell Lines
Expression Profile

Knock out of GALK2

Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III available from abm, Cat. No. TM003. To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255).
Carbon dioxide (CO2): 5%, Temperature: 37.0
QC

1) Confirmed GALK knockout through Western Blot analysis (Figure 5A) and sequencing (Figure S3). 2)Tested O-glycosylation levels through enzymatic activity on the substrate SIVmac239 gp120 via Western Blot and SDS gel analysis (Figure 5B).

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorUniversity of Miami
Documents


Supporting Protocol
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