gamma-secretase Deficient E-Cre-ERT2 Stable Expressing Rat1 (AD6 Clone 1) Cell Line

T61701x106 cells / 1.0 ml


DescriptionThis rat cell line expresses a chimeric molecule containing a delta E-like fragment of Notch1 fused to a vesicular stomatitis virus epitope the Cre recombinase and a modified hormone binding domain of the estrogen receptor. The delivery of Cre recombinase depends on any event including gamma-secretase processing that releases the intracellular domain of Notch1 from the membrane. Nuclear activity of the Cre-ER-T2 chimera depends on the presence of tamoxifen. This cell line was derived from clones deficient in gamma-secretase activity thus displaying impaired activity on Notch. Reporter construct for the recombinase activity consists of an antibiotic resistance gene (hygromycin). This cell line would be well-suited for research regarding the functions of the Notch receptor gamma-secretase enzyme activity and their involvement in cellular signalling.
SpeciesRat (R. norvegicus)
Tissue/Organ/Organ SystemEmbryo
Cell MorphologyFibroblast
ApplicationsFor Research Use Only
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileNeomycin resistant if not treated with tamoxifen. Hygromycin resistant if treated with tamoxifen.
Unit quantity1x106 cells / 1.0 ml
QC1) Immunofluorescence; 2) Northern blot; 3) DNA sequencing

Supporting Protocol




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