GFP Purified Protein

000033P50 μg


DescriptionAffinity purified recombinant enhanced green fluorescent protein (GFP) is a versatile tool that can be effectively used as a control to determine transfection efficiency, for microinjection into tissues and cells as well as for expression studies. GFP Purified Protein can be used as a standard for SDS gels, for Western Blots, and for fluorometry. This protein has a 6X Histidine Tag at its N-terminus and was purified using immobilized metal affinity chromatography (IMAC).
Transfection Reagents TypeProtein Molecule
Unit quantity50 μg
Storage ConditionThe lyophilized protein is stable for at least one year from date of receipt at -20°C. Upon reconstitution, this protein can be stored in working aliquots at 2°-8°C for one week. Avoid repeated freeze/thaw cycles.

Supporting Protocol




        Does freeze-thaw affect the performance of the transfection reagent?
        Freeze-thaw does damage the transfection reagent slightly, at an approximate 5% loss. If possible, test the agent with a GFP control first.
        Is this protein biologically active?
        The biological activity of this recombinant protein is not tested and therefore cannot be guaranteed. All downstream applications must be determined by the end user.
        What purity is this protein provided at?
        Our recombinant proteins are provided at >90% purity.
        What are the excitation and emission wavelengths of GFP purified protein?
        The predicted excitation wavelength is 488 nm and the predicted emission wavelength is 509 nm.

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