GFP1-10 Stable Expressing A549 Cell Line
|T6171||1x106 cells / 1.0 ml|
GFP1-10 Stable Expressing A549 Cell Line was established by transducing parental A549 cells with the pCMV-GFP1-10 plasmid. By itself, GFP1-10 is a fragment containing three residues that constitute the GFP chromophore, thus is non-fluorescent. Therefore, it is valuable cell line to be used in assays in which GFP11 is fused to an epitope of a target protein to study. The fused protein may be transfected into the cell line. Upon complementation, GFP1-10 and GFP11 fluoresces and can be visualized by microscopy.
|Species||Human (H. sapiens)|
|Seeding Density||30,000 - 35,000 cells/cm2|
For Research Use Only
|Unit quantity||1x106 cells / 1.0 ml|
|Cell Type||Drug Discovery Cell Lines|
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available
at?abm, Cat. No.?TM003. To make the complete growth medium, add the following
components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and
Penicillin/Streptomycin Solution (G255) to a final concentration of
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.
2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].
3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.
4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).
5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.
6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.
7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
- Beignon, AS et al. “Lentiviral vector-based prime/boost vaccination against AIDS: pilot study shows protection against Simian immunodeficiency virus SIVmac251 challenge in macaques” J Virol. 83(21):10963-10974 (2009). DOI: 10.1128/JVI.01284-09.
- Avilov, SV et al. “Replication-competent influenza A virus that encodes a split-green fluorescent protein-tagged PB2 polymerase subunit allows live-cell imaging of the virus life cycle” J Virol. 86(3):1433-1448 (2012). DOI: 10.1128/JVI.05820-11.