HEK293S-TetR GnTI- Stably Expressing TP Cell Line

CAT.NOUNITPRICE
T31631x106 cells / 1.0 ml
$0.00

Specifications


DescriptionHEK293S-TetR GnTI- Stably Expressing TP Cell Line was established to have a tetracycline-inducible HEK293S stable cell line which expresses the thromboxane A2 receptor (TP). TP is a G-protein coupled receptor (GPCR) involved in vasoconstriction and thrombosis. HEK293S-TetR GnTI- cells do not express the N-acetylglucosaminyltransferase I (GnTI). It is valuable tool for discovery of the TP biology, and the roles and functions of the receptor.

abm has available the A160T mutant of the TP receptor available (Cat. No. T3165).
SKUT3163
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemRenal
Growth PropertiesAdherent
Cell MorphologyPolygonal
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetGPC Receptors
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.

For membrane protein induction: Grow to 70-80% confluence and treat with tetracycline (1 ?g/ml) and sodium butyrate (7.5 mM) to induce the expression of the proteins. For optimum protein production concentration of both tetracycline and sodium butyrate need to be optimized.
QCImmunodetection was used to detect TP genetic variant A160T
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorUniversity of Manitoba
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      References


      1
      • Xu, B et al. "High-level expression, purification and characterization of a constitutively active thromboxane A2 receptor polymorphic variant" PLoS One. 8(9):e76481 (2013). DOI: 10.1371/journal.pone.0076481.