HeLa Overexpressing Stable tCD-Luc2 Cell Line

T60231x106 cells / 1.0 ml


DescriptionThe HeLa Overexpressing Stable tCD-Luc2 Cell Line contains the fusion gene tCD2-luc2 from the luciferase 2 protein and the rat CD2 protein. The overexpression of tCD2-luc2 in the HeLa cells was done by stable transfection using lentiviral infection with the plasmid DNA containing the fusion gene. The growth of this cell line is identical to the wild type cells which indicates that the exogenous protein tCD2-luc2 has no toxic effect. The HeLa Overexpressing Stable tCD-Luc2 Cell Line shows a significant amount of luciferase activity which produces bioluminescent cells. This is useful as an experimental tool of major importance for cancer investigation, especially imaging of tumors in xenografted animals where stable GFP, labelling of tumor cells have to be avoided.
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemReproductive
Growth PropertiesAdherent
Cell MorphologyPolygonal
Seeding Density10,000 - 20,000 cells/cm2
Population Doubling Time13 - 28 hours
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetReporters
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10% and 1% Penicillin/Streptomycin Solution.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
QC1) Flow cytometry on the basis of OX34 staining; 2) Western blot analysis of tCD2-luc2

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorGustave Roussy Transfert

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  • Jimenez, AS et al. "Rapid obtention of stable, bioluminescent tumor cell lines using a tCD2-luciferase chimeric construct" BMC Biotechnology 11(1):26 (2011). DOI: 10.1186/1472-6750-11-26.