Human T Lymphoblast Stably Deoxycytidine Kinase Deficient (AraC-8C) Cell Line

T31571x106 cells / 1.0 ml


DescriptionHuman T lymphoblasts are susceptible to HIV infection, and past studies had focused on the anti-HIV drug, 2‘,3‘-dideoxycytidine (ddC) for treatment. ddC relies on the nucleoside transport system and studies have linked the deoxycytidine kinase is involved in drug metabolism. The Human T Lymphoblast Stably Deoxycytidine Kinase Deficient Cell Line (AraC-8C) is a mutant clonal derivative of the CCRF-CEM cell line, which is also hypoxanthine-guanine phosphoribosyltransferase-deficient. AraC-8D cells are able to grow in the presence of 5 µM arabinosylcytosine and are deficient in nucleoside transport activities making it resistant to ddC unlike the parental cells. Additionally, the cells exhibit resistance to Ara-C, commonly used anti-cancer chemotherapy drug. It is valuable for research relating to the functions of nucleoside transport and related pathways, and for anti-cancer chemotherapeutic analysis.
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemBlood
Growth PropertiesSuspension
Cell MorphologyLymphoblast
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetKinases
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation RequirementsThe base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

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6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorOregon Health & Science University (OHSU)

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      • Ullman, B et al. "Dideoxycytidine metabolism in wild type and mutant CEM cells deficient in nucleoside transport or deoxycytidine kinase" Adv Exp Med Biol. 253:415-420 (1989). PubMed: 2558543.
      • Ullman, B et al. "Genetic analysis of 2',3'-dideoxycytidine incorporation into cultured human T lymphoblasts." J Biol Chem. 263(25):12391-12396. (1988). PubMed: 2842332.
      • Chottiner, EG et al. "Cloning and expression of human deoxycytidine kinase cDNA" Proc Natl Acad Sci U S A 88(4):1531-1535 (1991). PubMed: 1996353.