Huntingtin 150Q Stable PC12 Cell Line

CAT.NOUNITPRICE
T60181x106 cells / 1.0 ml
$0.00

Specifications


Description

Huntington disease (HD) is an autosomally dominant degenerative disorder resulting from polyglutamine expansion (>37units) in the huntingtin gene. The repeat is often localized in the N-terminal region of huntingtin and the resulting N-terminal fragments accumulate in the nucleus and become toxic to the cells. The Huntingtin 150Q Stable PC12 cell line is a rat pheochromocytoma cell line that expresses the N-terminal fragment of huntingtin containing 150 glutamine residues. The HD 150Q protein is predominantly present in the nuclei and as a result, these cells lack normal neurite development. Its control cell line, the Huntingtin 20Q Stable PC12 cell line, in contrast, displays the HD 20Q proteins evenly in the cytoplasm. The two cell lines together provide a valuable model for studies relating to altered gene expression caused by the polyglutamine tracts and the pathology of HD.
Generated by the co-transfection of a pCIS expression vector expressing a partial huntingtin cDNA containing 150 CAG repeats and a pCDNA3 expression vector carrying G418 resistance.

SKUT6018
SpeciesRat (R. norvegicus)
Species descriptionRattus norvegicus
Tissue/Organ/Organ SystemRenal
Growth PropertiesAdherent
Cell MorphologySmall|Irregular
Seeding Density30,000 - 50,000 cells/cm2
Population Doubling Time40 - 50 hours
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetOther
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements

Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 5%, horse serum to the final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorEmory University
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      References


      3
      • Xu, Q et al. "Synaptic Mutant Huntingtin Inhibits Synapsin-1 Phosphorylation and Causes Neurological Symptoms" The Journal of Cell Biology 202(7):1123–1138 (2013). DOI: 10.1083/jcb.201303146.
      • Li, SH et al. "Interaction of Huntington Disease Protein with Transcriptional Activator Sp1" Molecular and Cellular Biology 22(5):1277–1287 (2002). PubMed: 11839795.
      • Li, SH et al. "Cellular defects and altered gene expression in PC12 cells stably expressing mutant huntingtin" J Neurosci 19 (13) :5159-5172 (1999). PubMed: 10377328.