Immortalized Human Ovarian Epithelial Cells - SV40

Cat. No.
T1074
Unit
1x10⁶ cells / 1.0 ml
Price
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Cat. No. T1074
Name Immortalized Human Ovarian Epithelial Cells - SV40
Description

Real Time PCR was used to quantify SV40 gene expression in immortalized cell line

Organism Human (H. sapiens)
Tissue Ovary
Donor History Female
Growth Properties Adherent, polygonal cuboidal
Cell Type Immortalized Cells
Unit 1x10⁶ cells / 1.0 ml
Storage Condition Vapor phase of liquid nitrogen, or below -130°C.
Shipping Conditions Ship with dry ice.
Product Format Frozen
Intended Use This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
BioSafety II
Certificate of Analysis For batch-specific test results, refer to the applicable certificate of analysis that can be found at www.abmgood.com.
Growth Conditions

Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. PriGrow I (TM001) + 10% FBS + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂

Unpacking and Storage Instructions

1. Visually examine the packaging containers for signs of leakage or breakage.

2. Immediately transfer frozen cells from dry ice packaging to a temperature below -130°C, preferably in liquid nitrogen vapor phase storage, until ready for use.

To ensure the highest level of viability, thaw the vial and initiate culture as soon as possible upon receipt. If continued storage is desired, the vial should only be stored below -130°C or in liquid nitrogen vapor phase. Do not store at -70°C, as it will result in loss of viability.


Thawing Protocol

1. Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.

2. Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.

3. Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.

4. Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in the recommended pre-warmed, complete growth media and dispense into a T25 culture flask.

5. Incubate the cells at the recommended conditions.

Subculture Protocol

Volumes given below are for a T75 flask; proportionally increase or decrease the volume as required per culture vessel size. Subculture cells once the culture vessel is 80% confluent.

1. Aspirate the culture media, and add 2-3ml of pre-warmed 0.25% Trypsin-EDTA to the culture vessel.

2. Observe the cells under a microscope to confirm detachment (typically within 2-10 minutes). Cells that are difficult to detach can be put in 37°C, for several minutes to facilitate detachment.

3. Neutralize Trypsin-EDTA by adding an equal volume of the complete growth media into the culture vessel.

4. Transfer the culture suspension into a sterile centrifuge tube, and centrifuge at 125xg for 5 minutes. The actual centrifuge duration and speed may vary depending on the cell type.

5. Aspirate the supernatant, and re-suspend the pellet with pre-warmed fresh complete growth media. Add appropriate aliquots of the cell suspension to new culture vessels, as desired.

6. Incubate the cells at the recommended conditions.

Cryopreservation

Cryopreservation Medium (TM024), or complete growth media with 10% DMSO.

Seeding Density (cells/cm2) 15,000 - 30,000
Split Ratio 1:3 or 1:4
Population Doubling Time (h) 19 - 29
Immortalization Method Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Expression

CK18, CK19, CD31

STR Profiling

D5S818 : 10,12

D13S317 : 10,11

D7S820 : 9,10

D16S539 : 10,12

VWA : 15,18

TH01 : 8,9.3

AMEL : X,X

TPOX : 8,9

CSF1PO : 12,13

D12S391 : 18,23

FGA : 20,23

D2S1338 : 22,25

D21S11 : 29,32.2

D18S51 : 12,14

D8S1179 : 12,15

D3S1358 : 15,17

D6S1043 : 11,11

PENTAE : 7,18

D19S433 : 13,14

PENTAD : 11,13

D1S1656 : 14,15

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T1074.
Warranty abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
Disclaimer
1. All test parameters provided in the CoA are conducted using abm's standardized culture system and The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

2. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of order placement. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location.

3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic or any other non-RUO application(s).

4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature are provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

5. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period".
Application Research Use Only.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. T1074
Print & Download Datasheet
  • Kim, M., Rooper, L., Xie, J., Kajdacsy-Balla, A. A., & Barbolina, M. V. (2012). Fractalkine receptor CX(3)CR1 is expressed in epithelial ovarian carcinoma cells and required for motility and adhesion to peritoneal mesothelial cells. Molecular cancer research : MCR10(1), 11–24. https://doi.org/10.1158/1541-7786.MCR-11-0256


    Kim, M., Rooper, L., Xie, J., Rayahin, J., Burdette, J. E., Kajdacsy-Balla, A. A., & Barbolina, M. V. (2012). The lymphotactin receptor is expressed in epithelial ovarian carcinoma and contributes to cell migration and proliferation. Molecular cancer research : MCR10(11), 1419–1429. https://doi.org/10.1158/1541-7786.MCR-12-0361


    Wong, A. S., Choi, G. C., Cheng, A. A., Purcell, O., & Lu, T. K. (2015). Massively parallel high-order combinatorial genetics in human cells. Nature biotechnology33(9), 952–961. https://doi.org/10.1038/nbt.3326


    Kaplan, F., & Teksen, F. (2016). Apoptotic effects of salinomycin on human ovarian cancer cell line (OVCAR-3). Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(3), 3897–3903. https://doi.org/10.1007/s13277-015-4212-6 


    Johnson-Ajinwo, O. R., Richardson, A., & Li, W. W. (2019). Palmatine from Unexplored Rutidea parviflora Showed Cytotoxicity and Induction of Apoptosis in Human Ovarian Cancer Cells. Toxins, 11(4), 237.


    Liu, Q., Zhao, Y., Xing, H., Li, L., Li, R., Dai, J., … Fang, S. (2019). The role of R-spondin 1 through activating Wnt/β-catenin in the growth, survival and migration of ovarian cancer cells. Gene, 689, 124–130. https://doi.org/10.1016/j.gene.2018.11.098


    Rahman, M. A., Ramli, F., Karimian, H., Dehghan, F., Nordin, N., Ali, H. M., Mohan, S., & Hashim, N. M. (2016). Artonin E Induces Apoptosis via Mitochondrial Dysregulation in SKOV-3 Ovarian Cancer Cells. PloS one11(3), e0151466. https://doi.org/10.1371/journal.pone.0151466


    Zhang, R., Shi, H., Ren, F., Feng, W., Cao, Y., Li, G., ... & Zhang, M. (2019). MicroRNA-338-3p suppresses ovarian cancer cells growth and metastasis: implication of Wnt/catenin beta and MEK/ERK signaling pathways. Journal of Experimental & Clinical Cancer Research, 38(1), 1-13. doi: 10.1186/s13046-019-1494-3


    Buttarelli, M., De Donato, M., Raspaglio, G., Babini, G., Ciucci, A., Martinelli, E., … Gallo, D. (2020). Clinical Value of lncRNA MEG3 in High-Grade Serous Ovarian Cancer. Cancers, 12(4), 966. doi:10.3390/cancers12040966


    Hu, J., Wang, L., Zhao, W., Huang, Y., Wang, Z., & Shen, H. (2020). mi‑R4435‑2HG promotes proliferation and inhibits apoptosis of cancer cells in ovarian carcinoma by upregulating ROCK2. Oncology Letters, 19(2), 1305-1309.


    Jeffries, A. (2020). Role of the Novel DNS Sensors cGAS, IFI16, and ZBP1, During Viral Infections in Glia (Doctoral dissertation, The University of North Carolina at Charlotte).


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