IP6K1(-/-) & IP6K2(-/-) Stable HCT116 Cell Line

T63511x106 cells / 1.0 ml


DescriptionTo study the role that PP-IPs play in mammalian phosphate homeostasis researchers generated cells devoid of this small molecule. CRISPR was used to create a cell line without PP-IPs. IP6K3 is expressed mainly in muscle cells. Hence researchers chose human colon carcinoma cell line HCT116 as it is near-diploid and expresses IP6K1 and IP6K2 only and has easily detectable levels of IP7 and IP8. Researchers targeted IP6K1 and IP6K2 using guide RNAs against exon 5 of both genes to disrupt the inositol binding motifs. No IP6K1 or IP6K2 protein as well as IP7/IP8 was detectable in double knockout (DKO) IP6K1/IP6K2/ cell lines.
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemColon
Cell MorphologyEpithelial
ApplicationsFor Research Use Only
Seeding Density10,000 - 20,000 cells/cm2
Population Doubling Time20 - 30 hours
Donor GenderMale
Donor GenderMale
Donor AgeAdult
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileIP6K1 and IP6K2 knockout
Preservation Protocol1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) PCR; 2) Sequencing

Supporting Protocol




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