IP6K1(-/-) Stable HCT116 Cell Line

T63491x106 cells / 1.0 ml


DescriptionTo study the role that PP-IPs play in mammalian phosphate homeostasis researchers generated cells devoid of this small molecule. CRISPR was used to create a cell line without PP-IPs. IP6K1-/- line did not have significantly less IP7 than WT (wildtype). IP6K3 is expressed mainly in muscle cells and the cell lines tested were not muscle-derived and contained no or a very low amount of IP6K3 mRNA. Hence they chose human colon carcinoma cell line HCT116 as it is near-diploid and expresses IP6K1 and IP6K2 only and has easily detectable levels of IP7 and IP8.
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemColon
Cell MorphologyEpithelial
ApplicationsFor Research Use Only
Seeding Density30,000 - 40,000 cells/cm2
Population Doubling Time58 - 68 hours
Donor GenderMale
Donor GenderMale
Donor AgeAdult
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileIP6K1 knockout
Preservation Protocol1. Freeze Medium: 90% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) PCR; 2) Sequencing

Supporting Protocol




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