KCC2 Stable HEK293 Cell Line

CAT.NOUNITPRICE
T30381x106 cells / 1.0 ml
$0.00

Specifications


Description

Potassium-chloride transporter member 5 (SLC12A5, also known as KCC2) is a neuron-specific K-Cl symporter responsible for maintaining ion homeostasis in the central nervous system. The electrochemical chloride gradient established by KCC2 activity is critical in regulating postsynaptic inhibition, as well as in protecting cells from stimulation-induced excitotoxicity. The KCC2 Stable HEK293 cell line stably over-expresses the neuronal-specific K-Cl co-transporter isoform KCC2. In contrast to KCC1, where its activity is stimulated by osmotic swelling, KCC2 activity is increased when stimulated with NEM (N-ethylmaleimide). This cell line is suitable for central nervous system studies in the potential roles of KCC2 in ion homeostatsis.

SKUT3038
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemRenal
Growth PropertiesAdherent
Cell MorphologyPolygonal
Seeding Density30,000 - 50,000 cells/cm2, Split ratio: 1:2 or 1:3
Population Doubling Time30 - 40 hours
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetTransporters
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. To culture the cells in the presence of selection, add 2 µg/ml Puromycin (G264) to the complete media.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
QC

1) Cell line was screened using the 86Rb influx assay to determine KCC2 expressed at cell surface; 2) Western blot was used to examine protein expression

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorVanderbilt University
Documents


Supporting Protocol
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