Luciferase Stable U87MG/ΔEGFR Cell Line

CAT.NOUNITPRICE
T30091x106 cells / 1.0 ml
$0.00

Specifications


Description

Luciferase is commonly used as a reporter to assess the transcriptional activity of a genetic construct with luciferase gene under the promoter of interest. Luciferase stable was generated using abm's pLenti-III-UbC-Luciferase lentivirus on U87MG/ΔEGFR Cells. The expression of luciferase has been confirmed with luciferase assay. All clones are puromycin (0.7ug/ml)-resistant. Luciferase stable U87MG/ΔEGFR cells can be a very useful cell line for non-invasive visualization in in vitro experiments.

SKUT3009
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemBrain
Seeding Density10,000 cells/cm2
Population Doubling Time18 - 28 hours
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Propagation Requirements

The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Carbon dioxide (CO2): 5%, Temperature: 37.0°C.

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

Induction Method

Induced with lentivirus containing the LUCIFERASE (LUC2) Lentiviral Vector (Photinus pyralis) (UbC), Cat. No. LV010087

CautionFor Research Use Only
Cell TypeReporter Cells
FAQs


Should I culture this cell line in the presence (0.7 ug/ml) or absence of puromycin in the cell culture media ?
This cell line should be kept under a selection pressure to ensure that the cells do not lose the stable luciferase expression. It is recommended to use 0.2-0.5ug/ml puromycin for daily culture.
Can you describe the U87MG/deltaEGFR cell line?
U87MG/deltaEGFR cell line expresses a truncated form of epidermal growth factor receptor (EGFR) - Δ(2-7) EGFR (in-frame deletion of 801bp in exons 2-7, also known as type III mutation). U87MG/deltaEGFR expresses EGFRvIII (mutant epidermal growth factor receptor). The method used to generate this cell line is via Retroviral vector. U87MG/deltaEGFR also confers a high malignancy to tumour cells.
Is the GFP or RFP signal localized within the cell?
No, there is no localization tag on the GFP or RFP, so they can diffuse throughout the cell. However, if you want a localized signal, abm's Organelle Labeling Lentiviruses can be used for our Stable Cell Line Generation service. Simply ask [email protected] for a quote.
What type of GFP or RFP do these cell lines express, and from which promoter?
The GFP is derived from copGFP (from Pontellina plumata) and the RFP is derived from mKate2 (from Entacmaea quadricolor). They are expressed from a proximal SV40 promoter, and a more distal CMV promoter may also drive expression. If you are looking for a cell line with a specific fluorescent reporter, or with a specific promoter, abm can perform a Stable Cell Line Generation service. Simply ask [email protected] for a quote.
References


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