M2/GIRK1/GIRK4 Stably Expressing HEK293 Cell Line

CAT.NOUNITPRICE
T64721x106 cells / 1.0 ml
$0.00

Specifications


SKUT6472
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemKidney
Applications

For Research Use Only

Cell TypeDrug Discovery Cell Lines
Propagation Requirements
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow VII medium available at abm, Cat. No. TM017. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. To apply selective pressure, culture the cells in the presence of 727 µg/ml G418 (G262) and 364 µg/ml Zeocin.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Unit quantity1x106 cells / 1.0 ml
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Supporting Protocol
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      References


      3
      • Leaney, JL et al. "The G protein alpha subunit has a key role in determining the specificity of coupling to, but not the activation of, G protein-gated inwardly rectifying K(+) channels" J Biol Chem 275(2): 921-929 (2000). DOI: 10.1074/jbc.275.2.921.
      • Leaney, JL et al. "Rapid desensitization of G protein-gated inwardly rectifying K(+) currents is determined by G protein cycle" Am J Physiol Cell Physiol. 287(1): C182-C191 (2004). DOI: 10.1152/ajpcell.00540.2003.
      • Nobles M et al. "Differential effects of inhibitory G protein isoforms on G protein-gated inwardly rectifying K+ currents in adult murine atria" Am J Physiol Cell Physiol. 314(5): C616-C626 (2018). DOI: 10.1152/ajpcell.00271.2016.