OATP1B3 Stable Expressing HEK293 Cell Line
|T6239||1x106 cells / 1.0 ml|
|Description||A new organic anion transporting polypeptide (OATP) was cloned and expressed; deemed human OATP2. OATP plays an important role in the uptake of a variety of lipophilic anions into the human liver. HEK293 cells were transfected with the genes to express the recombinant OATP2 protein onto the lateral membrane of the cells. OATP1B3 Stable Expressing HEK293 Cell Line presents a good model to study the molecular mechanism behind the uptake of organic anions such as bilirubin conjugates and sulfobromophthalein into the human liver.|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Seeding Density||50,000 - 70,000 cells/cm2; cells may take up to 72 hours to recover post-thaw|
|Population Doubling Time||30 - 40 hours|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||OATP1B3 expression; G418 resistance|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) qRT-PCR; 2) Immunoblot; 3) Immunofluorescence analyses|
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