OATP1B3 Stable Expressing HEK293 Cell Line

T62391x106 cells / 1.0 ml


DescriptionA new organic anion transporting polypeptide (OATP) was cloned and expressed; deemed human OATP2. OATP plays an important role in the uptake of a variety of lipophilic anions into the human liver. HEK293 cells were transfected with the genes to express the recombinant OATP2 protein onto the lateral membrane of the cells. OATP1B3 Stable Expressing HEK293 Cell Line presents a good model to study the molecular mechanism behind the uptake of organic anions such as bilirubin conjugates and sulfobromophthalein into the human liver.
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemKidney
Cell MorphologyEpithelial
ApplicationsFor Research Use Only
Seeding Density50,000 - 70,000 cells/cm2; cells may take up to 72 hours to recover post-thaw
Population Doubling Time30 - 40 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileOATP1B3 expression; G418 resistance
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) qRT-PCR; 2) Immunoblot; 3) Immunofluorescence analyses

Supporting Protocol




      There are no FAQs for this product yet!

      There are no references for this product yet!