p47^(phox) Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line

CAT.NOUNITPRICE
T31811x106 cells / 1.0 ml
$0.00

Specifications


DescriptionImmortalization using polyoma middle-sized T-antigen (PmT) method Description: The p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line was derived from primary mouse aortic endothelial cells isolated from the thoracic and abdominal aortas of p47phox knockout mice. The primary cells were immortalized using polyoma middle-sized T-antigen (PmT) method. The p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line is able to retain the properties and phenotype of the parental cells including the expression of common markers of endothelial cells including PECAM1, eNOS, VE-cadherin, and von Willebrand Factor. Another interesting characteristic of this cell line is the decrease in superoxide production due to the lack of p47phox. When tested for the functional responses to physiologically relevant shear stresses, such as laminar shear, the p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line still maintained the ability to form tubes as expected from primary endothelial cells. With these characteristics, the p47phox Stable Knockout Mouse Aortic Endothelial (iMAEC-p47) Cell Line is a useful model in cardiology, particularly in studying vascular biology and pathobiology in vitro. It is also useful in studies on p47phox and its role in superoxide production.
SKUT3181
SpeciesMouse (M. musculus)
Species descriptionMouse
Tissue/Organ/Organ SystemHeart
Growth PropertiesAdherent
Cell MorphologyElongated
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetOther
CautionFor Research Use Only
Gene Knockdown MethodIsolated from genetically modified p47phox knockout mice
Cell TypeDrug Discovery Cells
Expression ProfilePECAM1, eNOS, VE-cadherin, and von Willebrand Factor
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 1% endothelial cell growth supplement (ECGS) crude extract and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Subculture Protocol1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Centrifuge cells at 1500rpm for 3 minutes to pellet. 4. Aspirate out trypsin, leaving pellet undisturbed. 5. Resuspend pellet in fresh culture medium and plate in new culture vessel. 6. Incubate cultures at 37°C.
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
QC1) FACS cell sorting; 2) Characterization by Dil-Ac-LDL staining and immunostaining
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

Documents


Supporting Protocol
MSDS

    QC

      Other

      FAQs


      There are no FAQs for this product yet!
      References


      1
      • Ni, CW et al. "Development of immortalized mouse aortic endothelial cell lines" Vascular Cell 6: (0). DOI: 10.1186/2045-824X-6-7.