PARP-1 Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line

CAT.NOUNITPRICE
T30311x106 cells / 1.0 ml
$0.00

Specifications


DescriptionPoly(ADP-ribose) polymerase 1 (PARP-1) is an ADP-ribosylating enzyme that plays a critical role in DNA damage repair, in addition to functioning as a context- specific regulator of transcription factors. Various pathophysiological conditions, such as reperfusion injury, endotoxic shock, diabetes and aging are associated with endothelial cell dysfunction mediated by PARP-1.

The PARP-1-/-Knockout Immortalized Mouse Heart Endothelial (HYKO6) Cell Line is derived from stable transformation of SV40 into primary mouse heart endothelial cells from mice deficient for PARP-1. This cell line is capable of morphologic differentiation in capillary-like structures (CLS), similar to its primary counterparts, as shown by branching and anastomosing cords of cell networks and tube formation after 12 hours culturing on Matrigel. Moreover, HYKO6 responds to cytokine stimulation and has been shown to exhibit increased VCAM-1 and E-selectin expression after TNFα stimulation, making this cell line an important tool in the elucidation of PARP-1 signaling pathway involved in endothelial cell dysfunction and molecular mechanisms controlling cell gene expression in response to inflammatory stimuli.
SKUT3031
SpeciesMouse (M. musculus)
Species descriptionMouse (C57BL/6J)
Tissue/Organ/Organ SystemHeart
Growth PropertiesAdherent
Cell MorphologyCobble-stone
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetOther
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Expression Profile

CD105, CD31, ICAM-2, VCAM-1, vWF

Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
QC

1) Flow cytometry was used to confirm the expression of endothelial cell markers such as CD105, CD31, ICAM-2, VCAM-1 and MHC Class I antigens; 2) Immunofluorescence staining was used to assess the vWF expression; 3) Western blot was used to evaluate the expression of SV40 T-antigen in the immortalized cell line.

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorIMIM
Documents


Supporting Protocol
MSDS

    QC

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    References


    1
    • Carrillo, A et al. "Establishment of an immortalized PARP-1-/- murine endothelial cell line: a new tool to study PARP-1 mediated endothelial cell dysfunction" J Cell Biochem 94(6):1163-74 (2005). PubMed: 15696577.