Pax6 Stable U251N Cell Line
|T6168||1x106 cells / 1.0 ml|
CRISPR-Cas9 was utilized to knockout PAX6 from the U251N cells to establish Pax6 KnockOut U251N Cell Line (2.10 KO cells). Knockout was verified via Western blot and immunocytochemistry. PAX6 KnockOut U251N Cell Line (2.10 KO) may be paired with the rescued Pax6 Stable U251N Cell Line (2.10 R), available at abm Cat. No. T6168, to study the effects of PAX6.
|Species||Human (H. sapiens)|
|Species description||Homo sapiens|
|Donor Gender||Donor Info Not Disclosed|
|Donor Age||Donor Info Not Disclosed|
|Donor Ethnicity||Donor Info Not Disclosed|
|Population Doubling Time||20 hours|
For Research Use Only
|Mammalian Selection Marker||Blasticidin|
|Unit quantity||1x106 cells / 1.0 ml|
|Cell Type||Drug Discovery Cell Lines|
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
1) Rescued cells selected under blasticidin pressure.
|Depositor||The Arctic University of Norway (UiT)|
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