Pax6 Stable U251N Cell Line

T61681x106 cells / 1.0 ml



CRISPR-Cas9 was utilized to knockout PAX6 from the U251N cells to establish Pax6 KnockOut U251N Cell Line (2.10 KO cells). Knockout was verified via Western blot and immunocytochemistry. PAX6 KnockOut U251N Cell Line (2.10 KO) may be paired with the rescued Pax6 Stable U251N Cell Line (2.10 R), available at abm Cat. No. T6168, to study the effects of PAX6.

SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemBrain
Donor GenderDonor Info Not Disclosed
Donor AgeDonor Info Not Disclosed
Donor EthnicityDonor Info Not Disclosed
Growth PropertiesAdherent
Cell MorphologyElongated
Population Doubling Time20 hours

For Research Use Only

Mammalian Selection MarkerBlasticidin
Unit quantity1x106 cells / 1.0 ml
Cell TypeDrug Discovery Cell Lines
Propagation Requirements
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0

Subculture Protocol

1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

1) Rescued cells selected under blasticidin pressure.
2) Inducible EGFP-PAX6 was done by 10 ng/ml of doxocyclin (Dox) (cat#D3447, Sigma), and visualization by green fluorescence and anti-PAX6 western blot.

DepositorThe Arctic University of Norway (UiT)

Supporting Protocol




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