Mycoplasma PCR Detection Kit

Cat. No.
100 rxn
Cat. No. G238
Name Mycoplasma PCR Detection Kit

Save when you buy abm's Mycoplasma PCR Detection Kit bundled with MycoAway™ Elimination Cocktail (Cat. No. G398). Click here and add this bundle to your cart to save instantly!

Enjoy superior performance at lower costs!

abm's Mycoplasma PCR Detection Kit (Cat. No. G238) offers highly specific and sensitive detection of 200+ strains of Mycoplasma in less than 2 hours. This bestselling kit is designed to minimize false positives, ensuring quick and reliable routine screening of cell cultures. A recent official publication by a national public health agency found that our kit out-performs Sigma and Lonza's kits while being 6X less expensive than ATCC's kit (Russell et al., 2020).

Mycoplasma species are highly undesirable, easily acquired, and notoriously difficult to detect, altering infected cells at the molecular level and leading to visible changes in cell morphology and growth characteristics. Timely detection of Mycoplasma in cell culture media is recommended in order to deter wide-spread contamination and save on the costly efforts of elimination. Our proprietary MasterMix formulation contains a gel loading dye, making it convenient for gel electrophoresis.

Kit Features:

  • No DNA isolation needed: Add cell culture media sample directly to PCR reaction
  • Rapid and Comprehensive: Detect 200+ Mycoplasma species in less than 2 hours
  • Highly Sensitive and Specific: Demonstrated the highest true positive and lowest false positive rates compared to competing kits
  • Easy-to-use: Includes ready-to-use primer mix and positive control
  • 6X less expensive per sample compared to competing kits (Russel et al., 2020)
  • Out-performs Sigma and Lonza's kits (Russel et al., 2020)
  • Combine with our bestselling Mycoplasma Elimination Cocktail (Cat. No. G398) to eliminate 70+ species of Mycoplasma


Customer Testimonial

"The mycoplasma detection kit we received from ABM worked very well. It was fast, easy to use, and, with the included controls, the results were very easy to interpret. We will purchase this kit again the next time we are in need of mycoplasma detection."

- Haylie Romero, University of California San Diego


An official publication by a national public health agency finds our kit out-performs Sigma and Lonza's kits:

Russell, B. J., Horiuchi, K., Velez, J. O., Goodman, C. H., & Johnson, B. W. (2020). Mycoplasma detection in a historical arbovirus repository: Commercial kit comparison and implications for improved repository management. Journal of virological methods, 276, 113769.


ISO 13485:2016 MDSAP Certified

ISO 13485:2016 MDSAP Certified
Our PCR Products are manufactured under a Quality Management System conforming with ISO 13485:2016 as certified by Intertek (a MDSAP recognized auditing organization).


Product Components Quantity
BlasTaq™ 2X PCR MasterMix
1.25 ml
Primer Mix
100 μl
Positive Control
250 μl
Nuclease-Free H2O
1.0 ml

Why test your cells for Mycoplasma?

Due to its small size, Mycoplasma can be present in your growth media without being visibly detected. This can cause significant errors in your experiments due to its effects on:

  • proliferation
  • metabolism
  • gene synthesis & processing
  • adhesion properties
  • and more

Many publishers now request Mycoplasma testing to be reported along with manuscript submissions to ensure accuracy in data reporting. Visit our learning resources center to read more about Mycoplasma contamination.

Unit 100 rxn
Storage Condition

Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. 


2X the points for PCR Enzymes, Buffers, Solutions, and more!

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G238
There are no references for this product yet!

verified customer

Submitted on: 2023-12-13 21:21:25

The reviewer received points from abmVacations+ Loyalty Program in thanks for sharing their results on the use of this product with the scientific community.