Mycoplasma PCR Detection Kit
|Name||Mycoplasma PCR Detection Kit|
Enjoy superior performance at lower costs!
abm's Mycoplasma PCR Detection Kit (Cat. No. G238) offers highly specific and sensitive detection of 200+ strains of Mycoplasmas in less than 2 hours. This bestselling kit is designed to minimize false positives, ensuring quick and reliable routine screening of cell cultures. A recent official publication by a national public health agency found that our kit out-performs Sigma and Lonza's kits while being 6X less expensive than ATCC's kit (Russell et al., 2020).
Mycoplasmas are highly undesirable, easily acquired, and notoriously difficult to detect, altering infected cells at the molecular level and leading to visible changes in cell morphology and growth characteristics. Timely detection of Mycoplasmas in cell cultures is recommended in order to deter wide-spread contamination and save on the costly efforts of elimination. Our proprietary MasterMix formulation contains a gel loading dye, making it convenient for gel electrophoresis.
Why test your cells for Mycoplasma?
Due to its small size, Mycoplasma can be present in your growth media without being visibly detected. This can cause significant errors in your experiments due to its effects on:
Many publishers now request Mycoplasma testing to be reported along with manuscript submissions to ensure accuracy in data reporting. Visit our learning resources center to read more about Mycoplasma contamination.
Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly.
|What suggestions can you provide for the gel electrophoresis step?|
Positive results will show a 370bp-550bp band so the PCR products can be ran on either a 1% or a 2% Agarose Gel with the proper corresponding DNA Markers. Please run the gel TAE Buffer with 100V for 20-25 minutes.
|Can samples be collected in advance and stored?|
Collected samples can be stored at 4°C for 3-5 days before testing. Please note, samples should be drawn from cells that have been in culture for at least 48-72 hours un-disturbed and are at least 80% confluent.
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