Ultra-Pure Phi29 DNA Polymerase

Cat. No.
E014
Unit
100 µl
Price
$152.00
Cat. No. E014
Name Ultra-Pure Phi29 DNA Polymerase
Unit 100 µl
Category Molecular Biology Enzymes and Kits
Description

Ultra-Pure Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Ultra-Pure Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates. The enzyme is subject to a rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA. Quality control involves a non-specific DNase Activity Assay and qPCR DNA Contamination Test with a TaqMan probe.

Application

• Rolling circle amplification (RCA)
• Multiple displacement amplification (MDA)
• Whole genome amplification (WGA)
• DNA template preparation for sequencing
• Protein-primed DNA amplification

Format General Enzyme supplied with 5X Reaction Buffer
Storage Buffer 50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM DTT, 100 mM NaCl, and 50% (v/v) Glycerol.
Storage Condition Store all components at -20°C.
Caution This product is distributed for laboratory research only. Not for diagnostic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E014
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