Lenti-CMV-Blank-CBh-RFP-2A-Puro Control Vector
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Product Name
Lenti-CMV-Blank-CBh-RFP-2A-Puro Control Vector -
Unit
1.0 µg -
Description
Empty versions of our most popular vectors to use as a control during your lentiviral experiments.
RFP variant in this vector is mKate2 (far red).
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Disclaimer
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Caution
Not for diagnostic use.
Print/Download Datasheet
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Supporting Protocol
Ordelheide, AM et al. "Nor-1, a novel incretin-responsive regulator of insulin genes and insulin secretion" Mol Metab 2(3):243-55 (2013). DOI: 10.1016/j.molmet.2013.06.003. PubMed: 24044104.
Lee, MS et al. "Exploitation of the Complement System by Oncogenic Kaposi's Sarcoma-Associated Herpesvirus for Cell Survival and Persistent Infection " PLOS Pathogens 10 (9):e1004412 (2014). DOI: 10.1371/journal.ppat.1004412. PubMed: 25254972. Application: Control Vector.
Lellahi, SM. "POU3f2 in human gliomas - Expression pattern and functional role" Bergen Open Research Archive Thesis: (2014).
Davoodian, N et al. "MicroRNA-122 Overexpression Promotes Hepatic Differentiation of Human Adipose Tissue-Derived Stem Cells" J. Cell. Biochem 115 (9):1582–1593 (2014). DOI: 10.1002/jcb.24822. PubMed: 24733606.
Chen, HH et al. "IRF2BP2 Reduces Macrophage Inflammation and Susceptibility to Atherosclerosis" Circ Res 8:671-683 (2015). DOI: 10.1161/CIRCRESAHA.114.305777.
Song, J et al. "miR-370 and miR-373 regulate the pathogenesis of osteoarthritis by modulating one-carbon metabolism via SHMT-2 and MECP-2, respectively" Aging Cell 5:826-837 (2015). DOI: 10.1111/acel.12363.
Martiskainen, H. "Polygenic Risk Scores, Transcriptomics, and Molecular Mechanisms of Alzheimer’s Disease Related Risk Genes" University of Eastern Finland: Dissertations in Health Sciences 306: (2015).
Vasudevan, A., Baruah, P. S., Smith, J. C., Wang, Z., Sayles, N. M., Andrews, P., ... & Storchová, Z. "Single chromosome gains can function as metastasis suppressors and metastasis promoters" bioRxiv 590547: (2019).
Huang, Z., Byrd, O., Tan, S., Hu, K., Knight, B., Lo, G., Taylor, L., Wu, Y., Berchuck, A., & Murphy, S. K. (2023). Periostin facilitates ovarian cancer recurrence by enhancing cancer stemness. Scientific Reports, 13(1). https://doi.org/10.1038/s41598-023-48485-8
ABM community
Verified customer
Asked on Jan 30 2026
Answer
abm lentiviral transfer vectors use the third generation lentivirus system. It is based on the inactivated HIV genome. Note that our lentivirus packaging plasmids cannot be integrated into the host and are transiently expressed.
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Mar 24 2025
Answer
We recommend using abm’s 2nd Generation Packaging System Mix (Cat. No. LV003) or 3rd Generation Packaging System Mix (Cat. No. LV053). abm’s lentiviral vectors have been tested and are compatible with Invitrogen’s packaging mix; we have not tested other suppliers and cannot guarantee compatibility.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Mar 24 2025
Answer
Higher MOI will provide more copies of the antibiotic resistance gene per cell. Cells containing multiple copies of the resistance gene can withstand higher antibiotic concentrations compared to those at lower MOIs. The concentration of antibiotic should be adjusted to a level that will cause selection for the desired population of transduced cells without going below the minimum antibiotic concentration you have established in your killing curve.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Jan 30 2026
Answer
MOI (Multiplicity of Infection) refers to the number of viral particles per cell used in the infection, e.g. an MOI of 5 indicates that there are five infectious units (IU) or transducing units (TU) for every cell. MOI is determined by calculating the numbers of viral particles added per well then divide this number by the number of cells seeded into the well. We also recommend transducing the cells with a range of MOIs as different cell types may require different MOIs for successful transduction.
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
MOI = Product Titer (IU/ml) x Virus Volume (ml) / Total Cell Number
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Jan 30 2026
Answer
The concentration must be optimized for each cell type. Typical selection amounts are around 0.1 - 0.5µg per ml.
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Mar 24 2025
Answer
The standard RFP used in most of our vectors is mkate2. It has an excitation wavelength of 588nm and emission wavelength of 633nm.
ABM Scientific Support
Answered on Mar 24 2025
ABM community
Verified customer
Asked on Jan 30 2026
Answer
These are medium-high copy plasmids and should be propagated in a cloning E. coli strain such as DH5α. Typical yields from a 250ml culture is 300-500µg plasmid DNA.
ABM Scientific Support
Answered on Jan 30 2026
ABM community
Verified customer
Asked on Jan 01 1970
Answer
This construct has AflII and AvrII sites. AflII is a 2 cutter for the vector and AvrII is a single cutter.
AflII will yield 2 bands at ~4.7kb and ~4.1kb.
AvrII will yield a single band at ~8.8kb.
If you cut with both of these enzymes, you may expect ~1.6kb, ~3.1kb, ~4.1kb bands.
ABM Scientific Support
Answered on Jan 01 1970
ABM community
Verified customer
Asked on Jan 01 1970
Answer
We have two lentiviral packaging systems that are available for researchers to use:
Second Generation - Higher Titer Capability - http://www.abmgood.com/2nd-Generation-Lentivirus-Packaging.html
Third Generation - Higher Safety Features - http://www.abmgood.com/3rd-Generation-Lentivirus-Packaging.html
ABM Scientific Support
Answered on Jan 01 1970
ABM community
Verified customer
Asked on Jan 30 2026
Answer
MOI stands for multiplicity of infection. Theoretically, an MOI of 1 will provide 1 virus particle for each cell on a plate, while an MOI of 10 represents ten virus particles per cell. However, several factors can influence the optimal MOI including cell line, cell type, transduction efficiency and gene of interest. We recommend first establishing an optimal MOI for each cell line. This can be done using a range of MOIs (0, 0.5, 1, 2, 5, 10, 50) to determine the MOI required to obtain optimal gene expression
ABM Scientific Support
Answered on Jan 30 2026
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Current vector selected:
Lenti-CMV-Blank-CBh-RFP-2A-Puro Control Vector
Cat. No.
LV591
Plasmid DNA Services
Midiprep Plasmid Amplification
10-20 µg
$75.00
Maxiprep Plasmid Amplification
80-100 µg
$150.00
Bacterial Agar Stab
1 stab
$40.00
Controls and Related Products
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
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AAV-CMV-Blank Control Vector
AAV001a
1 μg
$99.00
Empty
×
Current vector selected:
Lenti-CMV-Blank-CBh-RFP-2A-Puro Control Vector
Cat. No.
LV591
Controls and Related Products
DNAfectin™ Plus Transfection Reagent
G2500
1.0 ml
$245.00
Susfectin™ Transfection Reagent
G4000
1.0 ml
$245.00
AAViralEntry™ Transduction Enhancer (100X)
G516
1.0 ml
$190.00
AAV-CMV-Blank Control Vector
AAV001a
1 μg
$99.00
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