Poly(A) Polymerase, Yeast

Cat. No.
E017
Unit
100 U (100 μl)
Price
$52.50
Cat. No. E017
Name Poly(A) Polymerase, Yeast
Unit 100 U (100 μl)
Category Molecular Biology Enzymes and Kits
Description

Poly(A) Polymerase, Yeast catalyses the template independent addition of adenosine residues onto the 3' ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5'-triphosphate (3'-dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.

Application

• Labelling of RNA with ATP or cordycepin
• Poly(A) tailing of RNA for cloning or affinity purification
• Increasing translation of RNA transferred into eukaryotic cells

Concentration 1 U/μl
Components Enzyme supplied with 5X Reaction Buffer.
Storage Buffer 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol.
Storage Condition Store all components at -20°C.
Note

One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C.

Caution This product is distributed for laboratory research only. Not for diagnostic use .
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E017
Search CoA here
MSDS
There are no FAQs for this product yet!
  • Alghamdi, T.. "Insights into Glomerular Cell Biology in Health and Disease (Doctoral dissertation)" University of Toronto Toronto:Canada (2019).
  • Hou, W., Singh, R. K., Zhao, P., Martins, V., Aguilar, E., Canto, T., ... & Dias, A. C. P. "Transgenic expression of Hyp-1 gene from Hypericum perforatum L" alters expression of defense-related genes and modulates recalcitrance to Agrobacterium tumefaciens. Planta 251(1):13 (2020).
This product has no review yet.
Controls and Related Product:

CAT.NO
E017
UNIT
100 U (100 μl)
PRICE
$52.50

CAT.NO
G467
UNIT
80,000 U (200 μl)
PRICE
$152.00

CAT.NO
E049
UNIT
500 U (50 μl)
PRICE
$310.00

CAT.NO
E086
UNIT
20 Reactions
PRICE
$150.00

CAT.NO
D518
UNIT
50 preparations
PRICE
$299.00

CAT.NO
D516
UNIT
100 preparations
PRICE
$175.00

CAT.NO
D514
UNIT
100 Preparations
PRICE
$110.00

CAT.NO
E081
UNIT
50 Reactions
PRICE
$195.00