ProClone™ Competent Cells

CAT.NOUNITPRICE
E0035ml (4 x 1.25ml)
$120.00

Specifications


Description

ProClone? Competent Cells are high-efficiency, chemically competent DH5α (E. coli) cells that are optimized for use with abm’s versatile range of expression vectors. Transformation efficiency greater than 1x10^6 cfu/μg can be achieved using abm’s expression vectors, which are typically 3 to 4-fold larger in size and contain more complex genetic elements than the standard pUC19 plasmid. Reduced recombination and highly transformation efficiency make ProClone? Competent Cells the premier choice for both routine and challenging subcloning projects.

SKUE003
Unit quantity5ml (4 x 1.25ml)
Applications
  • transformation efficiency: >1x10^6 cfu/µg with abm’s expression vectors (>10kb in size). 
  • ideal for routine plasmid amplification. 
  • reduces recombination and improves yield and quality of plasmid DNA prepared from minipreps; can be sequenced directly. 
  • not suitable for preparing unmethylated DNA.
Expression SystemE. coli
Storage Condition

Store at -80°C.

Documents


Supporting Protocol
MSDS

    QC

      Other
      FAQs


      What is the genotype of these cells?
      F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 λ-
      References


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