R12A αA-crystallin Stable Expressing CHO Cell Line

CAT.NOUNITPRICE
T62341x106 cells / 1.0 ml
$0.00

Specifications


Description

R12A αA-crystallin Stable Expressing CHO Cell Line was designed to express the mutant R12A αA-crystallin. This cell line can be used to study the effects of the mutation on the protein's function as a molecular chaperone, an antiapoptotic protein, and as a structural protein of the lens.
αA-crystallin Stable Expressing CHO Cell Line (T6236) and R49A αA-crystallin Stable Expressing CHO Cell Line (T6235) are also available from abm.

SKUT6234
SpeciesChinese Hamster (C. griseus)
Tissue/Organ/Organ SystemOvary
Growth PropertiesAdherent
Cell MorphologyEpithelial-like
Seeding Density20,000 - 40,000 cells/cm2
Population Doubling Time20 - 30 hours
Applications

For Research Use Only

Mammalian Selection MarkerNeomycin
Unit quantity1x106 cells / 1.0 ml
Cell TypeDrug Discovery Cell Lines
Expression Profile

R12A αA-crystallin

Propagation Requirements

Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow XII medium available at abm, Cat. No. TM012. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. 400 µg/ml G418 (G271) was used for selection pressure.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Preservation Protocol1. Freeze Medium: 95% FBS and 5% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
QC

1) Western blot; 2) Apoptosis induction tests

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorCase Western Reserve University
FAQs


There are no FAQs for this product yet!
References


There are no references for this product yet!