Recombinant Human FGF2 (E. coli)

Cat. No.
Z101455
Unit
Price
$52.50
Cat. No. Z101455
Name Recombinant Human FGF2 (E. coli)
Description

Basic Fibroblast Growth Factor is found in almost all tissues of mesodermal and neuroectodermal origin and also in tumors derived from these tissues. Endothelial cells produce large amounts of this factor. Some bFGF is associated with the extracellular matrix of the subendothelial cells. Many cells express bFGF only transiently and store it in a biologically inactive form. The mechanism by which the factor is released by the cells is not known. It is released after tissue injuries and during inflammatory processes. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation. bFGF stimulates the growth of fibroblasts, myoblasts, osteoblasts, neuronal cells, endothelial cells, keratinocytes, chondrocytes, and many other cell types.

Organism Human (H. sapiens)
Unit quantity 50 µg
Family FGF Family
Aliases

HHT2, ALK1, HHT

Gene Symbol ACVRL1
Gene ID 1372
Accession Number NM_000020
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 17 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity

>95% as determined by SDS-PAGE

Formulation Lyophilized from a 0.2 μm filtered solution in PBS
Function The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.
Reconstitution A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL is recommended. Please note, filter sterilization is a must following reconstitution. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with 0.1% BSA without detectable loss of activity. Avoid repeated freeze/thaw cycles..
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101455
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