Recombinant Human IFNG (E. coli)

Cat. No.
Z100345
Unit
Price
$89.00
Cat. No. Z100345
Name Recombinant Human IFNG (E. coli)
Description IFNG is produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens and by lymphocytes expressing the surface antigens CD4 and CD8. Receptors for IFNG are expressed on all types of human cells with the exception of mature erythrocytes. IFNG-receptor complexes are rapidly internalized by endocytosis. In addition to its antiviral and antiparasitic activities, IFNG also inhibits the proliferation of a number of normal and transformed cells. The growth inhibitory activities of IFNG are in fact, more pronounced than those of the other interferons. However, the main biological activity of IFNG appears to be immunomodulatory in contrast to the other interferons that are mainly antiviral.
Organism Human (H. sapiens)
Unit quantity 100 µg
Family IL-10/Interferon Family
Aliases IFN-gamma, Immune interferon, IFNG, type II interferon, T cell interferon, MAF
Recombinant Human Interferon-Gamma (IFNG)
Gene Symbol IFNG
Gene ID 3458
Accession Number P01579
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 17 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in sodium phosphate and NaCl (pH 8.0)
Function The ED50 was found to be ≤ 0.5ng/ml as determined by its ability to induce dose dependent proliferation in vascular smooth muscle cells.
Reconstitution A quick spin of the vial followed by reconstitution in 20mM acetic acid not less than 0.1 mg/mL.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100345
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