RFP Stably Expressing REH Cell Line
|T3947||1x106 cells / 1.0 ml|
|Description||REH cells stably expressing RFP|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Unit quantity||1x106 cells / 1.0 ml|
|Propagation Requirements||T25 (B909) or T75 (B908) filter flasks are recommended for culturing this cell line. The base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the completed growth medium, add the following components to the base medium with the final concentration: heat inactivated fetal bovine serum to a final concentration of 10%, 0.2|
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.
|Cell Type||Reporter Cells|
|Is the GFP or RFP signal localized within the cell?|
|What type of GFP or RFP do these cell lines express, and from which promoter?|
The GFP is derived from copGFP (from Pontellina plumata) and the RFP is derived from mKate2 (from Entacmaea quadricolor). They are expressed from a proximal SV40 promoter, and a more distal CMV promoter may also drive expression. If you are looking for a cell line with a specific fluorescent reporter, or with a specific promoter, abm can perform a Stable Cell Line Generation service. Simply ask [email protected] for a quote.
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