Rieske ISP Stable Knockout Mouse Fibroblast Cell Line

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T63041x106 cells / 1.0 ml
$0.00

Specifications


DescriptionTo generate the Rieske ISP Stable Knockout Mouse Fibroblast Cell Line; primary lung fibroblast culture was established from a knockin mouse homozygous for the floxed UQCRFS1 (exon 2) gene. UQCRFS1 encodes for the Rieske iron sulfur protein subunit of complex III. Cells were immortalized by retroviral transduction with E6/E7 type 16 papiloma virus carrying G418 resistance. Clones were obtained from immortalization. One of the clones #8 was used to generate the RIESKE KO cells by transfecting with a plasmid expressing cre recombinase. Clones were obtained after hygromycin selection.
SKUT6304
SpeciesMouse (M. musculus)
Tissue/Organ/Organ SystemLung
Cell MorphologyFibroblast
ApplicationsFor Research Use Only
Population Doubling Time24 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileG418 and hygromycin-resistant
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) Western blot
Documents


Supporting Protocol
MSDS

    QC

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