Rieske ISP Stable Knockout Mouse Fibroblast Cell Line
|T6304||1x106 cells / 1.0 ml|
|Description||To generate the Rieske ISP Stable Knockout Mouse Fibroblast Cell Line; primary lung fibroblast culture was established from a knockin mouse homozygous for the floxed UQCRFS1 (exon 2) gene. UQCRFS1 encodes for the Rieske iron sulfur protein subunit of complex III. Cells were immortalized by retroviral transduction with E6/E7 type 16 papiloma virus carrying G418 resistance. Clones were obtained from immortalization. One of the clones #8 was used to generate the RIESKE KO cells by transfecting with a plasmid expressing cre recombinase. Clones were obtained after hygromycin selection.|
|Species||Mouse (M. musculus)|
|Applications||For Research Use Only|
|Population Doubling Time||24 hours|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||G418 and hygromycin-resistant|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Western blot|
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