RNase A
Cat. No.
G117
Unit
25 mg
Price
$55.00
| Cat. No. | G117 |
| Name | RNase A |
| Unit | 25 mg |
| Category | Molecular Biology Enzymes and Kits |
| Description |
RNase A is pancreatic bovine endoribonuclease that cleaves single stranded RNA by hydrolysis at cytosine and uracil residues. RNase A is very useful for the removal of RNA from plasmid DNA preparations. |
| Application |
• RNA removal from plasmid DNA preparations • RNA removal during DNA isolation • RNA sequence analysis • RNase protection assays |
| Concentration | A stock solution is recommended to be prepared in water at 10 mg/ml. Final usage concentration should be determined by end user based on specific application and usage. |
| Storage Condition |
Store all components at -20°C . |
| Note |
This product is distributed for laboratory research only Caution: Not for diagnostic use. |
Datasheet
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MSDS
| What is the medium of dissolving the powder (RNase)? | |
|
We dissolve RNAse A in ddH2O to make stock solution of 1mg/ml. We do not have a special protocol for dissolving RNAses in other buffers.
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| After dissolving your RNase A (powder) in solution, should I boil it at 100 C for 15-20 minutes to inactivate DNase contamination? | |
|
In order to remove any remaining DNase activity in the solution without compromising RNase A activity, we would suggest boiling the solution for 15 – 20 minutes. This is sufficient for most downstream molecular biology work e.g. preparation of RNA free plasmid DNA.
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