Ryanodine Receptor 1 Stable HEK293 Cell Line

CAT.NOUNITPRICE
T30371x106 cells / 1.0 ml
$350.00

Specifications


DescriptionRyanodine receptors (RyR) are intracellular calcium channels located in the sarcoplasmic reticulum and the endoplasmic reticulum, and they mediate the release of calcium ions during muscle contractions. RyR are structurally and functionally similar to the inositol 1,4,5-triphosphate receptors (IP3R), which is responsible in initiating intracellular calcium signaling pathways. Mutations to the receptor have been observed in numerous diseases states. The Ryanodine Receptor 1 Stable HEK293 Cell Line stably expresses functional RyR1 calcium release channels, thus providing a valuable tool for pharmacological investigations in the function and mechanisms of calcium release channels activity, and in depth biophysical studies of the RyR1 receptor.
SKUT3037
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemRenal
Growth PropertiesAdherent
Cell MorphologyPolygonal
Seeding Density30,000 - 60,000 cells/cm2
Population Doubling Time36 - 46 hours
Applications

For Research Use Only

Mammalian Selection MarkerHygromycin B
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetIon Channels
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to a final concentration of 10%, 100
Subculture Protocol1. Remove and discard culture medium. 2. Add 2.0mL of Trypsin-EDTA(TM050) solution to flask and observe cells under an inverted microscope until cell layer is dispersed. Note: Cells that are difficult to detach may be placed at 37
Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
QC

Western blot and immunofluorescence analysis was used to confirm the expression of RyR1

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorUniversity of Rochester
Documents


Supporting Protocol
MSDS

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    References


    3
    • Wagner , LE et al. "Characterization of ryanodine receptor type 1 single channel activity using "on-nucleus" patch clamp" Cell Calcium 56(2):96-107 (2014). DOI: 10.1016/j.ceca.2014.05.004. Epub 2014 Jun 6.
    • Zhao, F et al. "Dantrolene inhibition of ryanodine receptor Ca2+ release channels. Molecular mechanism and isoform selectivity" J Biol Chem 276(17):13810-13816 (2001). PubMed: 11278295.
    • Ward, CW et al. "Type 1 and type 3 ryanodine receptors generate different Ca(2+) release event activity in both intact and permeabilized myotubes" Biophys J 81(6):3216-3230 (2001). PubMed: 11720987.