Safe-Green™

Cat. No.
G108-G
Unit
1.0 ml
Price
$45.00
Cat. No. G108-G
Name Safe-Green™
Unit 1.0 ml
Category SafeView™ DNA Stains
Description

Safe-Green™ is a new and safe nucleic acid stain for the visualization of nucleic acids in agarose and polyacrylamide gels. This dye eliminates the need for toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis.

Features:

  • Convenient: Safe-Green™ is provided as a 6X loading dye, and is mixed directly with samples before gel loading. Inert tracking dye is included to monitor gel progress.
  • Easy to Use: View and document your results as you would with EtBr staining. Safe-Green™ can be excited with blue or UV light, and has maximum emission at 525 nm.
  • Safe: Non-carcinogenic.
  • Sensitive: Detect as little as 0.2 – 0.6 ng of DNA per gel band.
  • Superior: EtBr is known to cause strand breaks and nicks in DNA. Using Safe-Green™ minimizes such damage, yielding higher transformation rates and lower mutation rates verses EtBr. For even better cloning results use Safe-Green™ with blue light excitation.
Safeview Series Loading Dye
LED Viewer Compatibility Yes
Stain Color Green
Application

Safe detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.

Shipping Conditions

Shipped on blue ice packs.

Storage Condition

Store at 4°C for up to 2 years.  

Note

All SafeView DNA Stains are ISO-13485 certified.

Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G108-G
  • Ibeagha-Awemu, EM et al. "Proteomics, genomics, and pathway analyses of Escherichia coli and Staphylococcus aureus infected milk whey reveal molecular pathways and networks involved in mastitis" J. Proteome Res. 9 (9):4604-4619 (2010). DOI: 10.1021/pr100336e. PubMed: 20704270. Application: PCR Products Viewing.
  • Diaz-Balzac, CA et al. "Calbindin-D32k is localized to a subpopulation of neurons in the nervous system of the sea cucumber Holothuria glaberrima (Echinodermata)" PLoS ONE 7 (3):e32689 (2012). DOI: 10.1371/journal.pone.0032689. PubMed: 22412907. Application: PCR Products Viewing.
  • Machida, RJ et al. "PCR primers for metazoan nuclear 18S and 28S ribosomal DNA sequences" PLoS ONE 7 (9):e46180 (2012). DOI: 10.1371/journal.pone.0046180. PubMed: 23049971. Application: PCR Products Viewing.
  • Kim, NH et al. "Reactive oxygen species regulate context-dependent inhibition of NFAT5 target genes" Exp. Mol. Med. 45:e32 (2013). DOI: 10.1038/emm.2013.61.. PubMed: 23867654. Application: PCR Products Viewing.
  • Goo, JS et al. "Bacillus thuringiensis: a specific gamma-cyclodextrin producer strain" Carbohydr. Res. 07-Dec:386 (2014). DOI: 10.1016/j.carres.2013.12.005. PubMed: 24456970. Application: PCR Products Viewing.
  • Pyo, JS et al. "Activation of nuclear factor-κB contributes to growth and aggressiveness of papillary thyroid carcinoma" Pathol. Res. Pract. 209 (4):228-232 (2013). DOI: 10.1016/j.prp.2013.02.004. PubMed: 23528368. Application: PCR Products Viewing.
  • Ish-Shalom, S et al. "Analysis of fungal gene expression by Real Time quantitative PCR" Methods Mol. Biol. 638:103-114 (2010). DOI: 10.1007/978-1-60761-611-5_7. PubMed: 20238263. Application: PCR Products Viewing.
  • Lam, SW et al. "Rapid, specific and quantitative polymerase chain reaction (PCR) detection of pathogenic protozoa Entamoeba histolytica for drinking water supply" Water Science & Technology: Water Supply 11 (4):418-425 (2011). DOI: 10.2166/ws.2011.057. PubMed: 67657423. Application: PCR Products Viewing.
  • Chang, KF et al. "First report of Fusarium proliferatum causing root rot in soybean (Glycine max L.) in Canada" Crop Prot 67:52-58 (2015). DOI: 10.1016/j.cropro.2014.09.020. Application: PCR Products Viewing.
  • Mun, Y. S., & Hwang, Y. J. "Novel spa and Multi-Locus Sequence Types (MLST) of Staphylococcus Aureus Samples Isolated from Clinical Specimens in Korean" Antibiotics 8(4):202 (2019). DOI: 10.3390/antibiotics8040202.
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