Stable VERO Overexpressing HER2 and HER3 Cell Line

CAT.NOUNITPRICE
T31911x106 cells / 1.0 ml
$0.00

Specifications


DescriptionBoth HER2 and HER3 have been associated with the progression of cancer, including breast cancer. Stable VERO Overexpressing HER2 and HER3 Cell Line stably express full length HER2 and HER2. Co-expression of the receptors was established to study heterodimerization. The cells are valuable for studies in breast cancer research to bring insight in the development and progression.

abm has the Stable VERO Overexpressing HER2 Cell Line (T3189) and Stable VERO Overexpressing HER3 Cell Line (T3190) for experimentation with the HER2 and HER3 separately.
SKUT3191
SpeciesMonkey (Primate)
Species descriptionCercopithecus aethiops (African Green Monkey)
Tissue/Organ/Organ SystemRenal
Growth PropertiesAdherent
Cell MorphologyEpithelial-Like
Seeding Density20,000 cells/cm2
Population Doubling Time15 - 25 hours
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetKinases
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow VII medium available at abm, Cat. No. TM017. To make the completed growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 200 ug/mL G418 (G271), 200 ug/ml hygromycin B (G265), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
QC1) 500ug/mL G418 and 500ug/mL hygromycin used for selection; 2) Expression of HER2 and HER3 identified via immunoblotting
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorMazandaran University of Medical Sciences
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      References


      1
      • Hedayatizadeh-Omran, et al. "VERO stable cell lines expressing full-length human epidermal growth factor receptors 2 and 3: platforms for subtractive phage display" DNA and Cell Biology 34(9):573-578 (2015). DOI: 10.1089/dna.2015.2917.