TLR9 Knockout Immortalized Mouse Dendritic Cell Line

CAT.NOUNITPRICE
T30351x106 cells / 1.0 ml
$0.00

Specifications


Description

As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The MutuDCTlr9-/- cell line is an immortalized splenic CD8α+ subset (CD11chigh,B220-,DEC205+,CD24high,CD11b-) of conventional dendritic cells derived from the TLR9 knockout CD11c:SV40LgT transgenic mice.
The MutuDCTlr9-/- cell line retains response to TLR ligands such as PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) but not CpG (TLR9-L). Like the Wild-type MutuDC cells (Cat. No. T0528), these cells are capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. Together with TLR3 knockout (Cat. No. T3034) and Ifnar1 knockout (Cat. No. T3036) MutuDC, these dendritic cell lines provide a powerful tool in vaccine science and immunotherapy, particularly in strategizing target antigens to CD8α+ subset.

SKUT3035
SpeciesMouse (M. musculus)
Species descriptionMouse (C57BL/6J)
Tissue/Organ/Organ SystemSpleen
Growth PropertiesAdherent
Cell MorphologySmall Aggregates
Seeding Density20,000 - 50,000 5 cells/cm2, should not be split lower than 5 x 104 cells per cm2
Population Doubling Time50 - 60 hours
Applications

For Research Use Only

Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetCatalytic Receptors
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Expression Profile

CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4

Propagation Requirements

The base medium for this cell line is PriGrow V available at abm (TM015). To make the completed growth medium, add the following components to the base medium: 10% decomplemented fetal calf serum (PAN biotech; P40-37500), 50 µM ?-mercaptoethanol, 1% HEPES, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Stimulation can be performed using CpG (2 mM) or LPS (5 µg/ml). Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
IMPORTANT: This cell line is very sensitive to FBS batches. We strongly recommend end users to use FBS obtained from PAN biotech.

Preservation Protocol1. Freeze Medium: Complete growth medium with 50% decomplemented fetal calf serum (PAN biotech; P40-37500) and 10% DMSO.

2. Storage Temperature: Liquid nitrogen vapour phase.
QC

1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA ; 4) Response to PAMP stimulation evaluated by functional assays.

Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.
2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]
3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5

DepositorUniversity of Lausanne
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References


1
  • Fuertes Marraco, SA et al. "Novel murine dendritic cell lines: a powerful auxiliary tool for dendritic cell research" Front Immunol 3:331 (2012). DOI: 10.3389/fimmu.2012.00331.