TrkA Stable T17 Cell Line

CAT.NOUNITPRICE
T31581x106 cells / 1.0 ml
$0.00

Specifications


DescriptionTrkA Stable T17 Cell Line expresses rat TrkA (Tropomyosin receptor kinase A) after TrkA plasmid was successfully transfected into SN56 cells and were selected in the presence of G418. TrkA is the receptor for neurotrophins, such as nerve growth factor (NGF), which plays essential role in the nervous system – in particular apoptotic cell death prevention, and/or the promotion of cellular differentiation and axon elongation in neurons. Researchers may use the TrkA Stable T17 Cell Line to screen for NGF-like compounds that would act as TrkA agonists to study the mechanisms of the signaling pathways and affects NGF. The lack of TrkA has been associated with the neurodegenerative processes and thus the cell line is a valuable tool to conduct related studies.

abm has available the TrkB Stable T48 Cell Line (T3159), which can be used to screen BDNF-like (brain-derived neurotrophic factor –like) compounds as well.
SKUT3158
SpeciesMouse (M. musculus)
Species descriptionMouse
Tissue/Organ/Organ SystemBrain
Growth PropertiesAdherent
Cell MorphologyPolygonal
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetKinases
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium to final concentration of fetal bovine serum (TM999) to a final concentration of 10%, 1 mM Sodium Pyruvate Solution(TM057), Penicillin/Streptomycin Solution (G255) to a final concentration of 1%, and 300 mg/ml G418 (G271).
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
QCSelection with 300 mg/ml G418
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorEmory University
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      References


      3
      • Obianyo, O et al. "Novel Small Molecule Activators of the Trk Family of Receptor Tyrosine Kinases" Biochim Biophys Acta 1834(10):2213–2218 (2014). DOI: 10.1016/j.bbapap.2012.08.021.
      • Jang, S et al. "Gambogic amide, a selective agonist for TrkA receptor that possesses robust neurotrophic activity, prevents neuronal cell death" Proceedings of the National Academy of Sciences 104(41):16329-16334 (2007). DOI: 10.1073/pnas.0706662104.
      • Jang, S et al. "The Antidepressant Amitriptyline is a TrkA and TrkB Receptor Agonist that Promotes TrkA/TrkB Heterodimerization and Has Potent Neurotrophic Activity" Chem Biol 16(6):644–656 (2009). DOI: 10.1016/j.chembiol.2009.05.010.