TSPO Stable Knockdown Huh7 Cell Line
|T6402||1x106 cells / 1.0 ml|
|Description||TSPO plays a critical role in the segregation and transport of free cholesterol (FC) from the outer to the inner mitochondrial membrane in cells. Wild type Huh7 (WT) is a well-differentiated human hepatocyte-derived carcinoma cell line which contains TSPO in mitochondria. The stable TSPO knockdown (TSPO KD) in the Huh7 cell line was made to mimic hepatic steatosis in vitro.|
|Species||Human (H. sapiens)|
For Research Use Only
|Seeding Density||80,000 - 100,000 cells/cm2|
|Population Doubling Time||24 hours|
|Cell Type||Drug Discovery Cell Lines|
Vector information: PBR shRNA plasmid (TSPO KD) Drug resistance: Puromycin (2ug/ml)
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
1) Western blot
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