TSPO Stable Knockdown Huh7 Cell Line

CAT.NOUNITPRICE
T64021x106 cells / 1.0 ml
$0.00

Specifications


DescriptionTSPO plays a critical role in the segregation and transport of free cholesterol (FC) from the outer to the inner mitochondrial membrane in cells. Wild type Huh7 (WT) is a well-differentiated human hepatocyte-derived carcinoma cell line which contains TSPO in mitochondria. The stable TSPO knockdown (TSPO KD) in the Huh7 cell line was made to mimic hepatic steatosis in vitro.
SKUT6402
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemLiver
Cell MorphologyEpithelial
Applications

For Research Use Only

Seeding Density80,000 - 100,000 cells/cm2
Population Doubling Time24 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression Profile

Vector information: PBR shRNA plasmid (TSPO KD) Drug resistance: Puromycin (2ug/ml)

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC

1) Western blot

Documents


Supporting Protocol
MSDS

    QC

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