Type I IFN Receptor Knockout Immortalized Mouse Dendritic Cell Line
|T3036||1x106 cells / 1.0 ml|
|Description||As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The MutuDC1IFNR -/-) cell line is an immortalized splenic CD8α subset (CD11chigh,B220-,DEC205 ,CD24high,CD11b-) of conventional dendritic cells derived from the 1IFNR knockout CD11c:SV40LgT transgenic mice. |
Like the Wild-type MutuDC cell line (Cat. No. T0528), the MutuDC1IFNR -/- cell line retains response to TLR ligands such as CpG (TLR9-L) and PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens. Studies has shown that several genes were found to be differentially negatively (Tnfa and Pdl1) or positively (Ifnb, Il10, and Ccr7) upregulated in the MutuDC1IFNR -/- cells upon stimulation. Together with TLR3 knockout (Cat. No. T3034) and TLR9 knockout (Cat. No. T3035) MutuDC, these dendritic cell lines provide a powerful tool in vaccine science and immunotherapy, particularly in strategizing target antigens to CD8α subset.
|Species||Mouse (M. musculus)|
|Species description||Mouse (C57BL/6J)|
|Cell Morphology||Small Aggregates|
|Seeding Density||2.5 x 105 cells/cm2, should not be split lower than 5 x 104 cells per cm2|
|Applications||For Research Use Only|
|Unit quantity||1x106 cells / 1.0 ml|
|Pharmaceutical Target||Catalytic Receptors|
|Caution||For Research Use Only|
|Cell Type||Drug Discovery Cells|
|Expression Profile||CD11c, CD24, MHC-II+, DEC205, Clec9A, B220, CD11b, IRF4, IRF8, CD4|
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.The base medium for this cell line is PriGrow V available at abm (TM015). To make the completed growth medium, add the following components to the base medium: 10% decomplemented fetal calf serum (PAN biotech; P40-37500), 50 µM ?-mercaptoethanol, 1% HEPES, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%. Stimulation can be performed using CpG (2 mM) or LPS (5 µg/ml). Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
IMPORTANT: This cell line is very sensitive to FBS batches. We strongly recommend end users to use FBS obtained from PAN biotech.
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 50% decomplemented fetal calf serum (PAN biotech; P40-37500) and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|QC||1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA ; 4) Response to PAMP stimulation evaluated by functional assays.|
1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.
|Depositor||University of Lusanne|
- Fuertes Marraco, SA et al. "Novel murine dendritic cell lines: a powerful auxiliary tool for dendritic cell research" Front Immunol. 3:331 (2012). DOI: 10.3389/fimmu.2012.00331.