Meet the Next Generation of PCR Enzymes
abm is constantly evolving and so is our product line. Our pursuit of next generation PCR enzymes has led to intensive R&D and significant upgrades to our PCR products. The result - a new collection of world leading PCR enzymes and formulations with performance far beyond the current leading brands. Best of all - at the same discount prices we always offer.
Whether it's for research or diagnostic product development, we are confident our premium enzymes will meet all your project needs. With abm, you will get wholesale discount pricing without the membership fees - what are you waiting for? Try our products today and take your PCR to the next level!
MegaFi™ Fidelity
BlasTaq™
BlasTaq™ HotStart
MegaFi™ Fidelity DNA Polymerase
MegaFi™ Fidelity DNA Polymerase is the best high fidelity DNA Polymerase on the market. With 1300X better fidelity than regular Taq, this ultrasensitive polymerase amplifies even the most difficult templates.
1300X higher fidelity than Taq
Amplifies difficult templates
Effective in long-range PCR
Ultra-Low Error Rates
MegaFi™ DNA Polymerase is THE BEST high fidelity DNA polymerase. A lacZa-containing sequence was amplified using various DNA polymerases, subcloned, and plated on X-gal-containing media. Colonies without PCR amplified lacZa mutations appear dark blue, while those that have PCR introduced frameshift mutations appear white.
Superior Fidelity
MegaFi™ DNA Polymerase has ~1300X higher fidelity than other enzymes on the market. abm’s MegaFi™ Fidelity DNA Polymerase (Cat. No. G896) has the highest accuracy rate compared to other DNA polymerases as tested using blue/white colony screening. Shown as relative fidelity compared to Taq DNA Polymerase (Taq = 1X). PCR cycle number and mutations resulting in non-phenotypic changes are accounted for.
| PRODUCT | CAT. NO. |
|---|---|
| MegaFi™ Fidelity DNA Polymerase | G896 |
| MegaFi™ Fidelity 2X PCR MasterMix | G897 |
| MegaFi™ One-Step RT-PCR * | G597 |
| *Contains OneScript™ Hot Reverse Transcriptase (Cat. No. G593) | |
BlasTaq™ DNA Polymerase
Blast through a routine 2 hour PCR run in only 20 minutes with our BlasTaq™ DNA Polymerase. Reach extension speeds as fast as 6 kb/min and streamline your PCR!
6 kb/min extension speed
40-70% less time than Taq
Superior sensitivity
Super Fast Extension Speeds
BlasTaq™ DNA Polymerase enables extension speeds as fast as 6 kb/min. Total reaction times for abm’s BlasTaq™ DNA Polymerase (Cat. No. G894) and Taq were determined for the amplification of different sized amplicons: 500 bp, 1 kb, 2 kb, and 5 kb. Reaction times are based on a 30-cycle program using the recommended reaction protocol for each enzyme.
Highly Robust
PCR amplification using abm’s BlasTaq™ DNA Polymerase (Cat. No. G894) (column 1) of various targets vs. competitor polymerases (column 2 and 3) followed by electrophoresis on a 1% agarose gel. A) 1 kb target from Human cDNA. B) 1.3 kb target from plasmid DNA. C) 7.6 kb target from plasmid DNA.
BlasTaq™ HotStart DNA Polymerase
Eliminate non-specific amplification and primer-dimer formation with our next generation BlasTaq™ HotStart DNA Polymerase. This formulation includes a proprietary antibody that blocks polymerase activity at low temperatures and dissociates at the denaturation step to restore enzyme activity.
No non-specific amplification or primer dimers
Improves yield of desired product
Simplifies reaction setup
Eliminates Non-specific Amplification
BlasTaq™ HotStart DNA Polymerase eliminates non-specific amplification. abm ’s BlasTaq™ HotStart DNA Polymerase (Cat. No. G595) was used to amplify a 7 kb target. The antibody concentration was decreased incrementally from lanes 1-5. Lane 1 is our BlasTaq™ HotStart formulation and lane 5 contains no antibody.
Super Fast Extension Speeds
BlasTaq™ HotStart DNA Polymerase has extension speeds of 6 kb/min. abm ’s BlasTaq™ HotStart DNA Polymerase (Cat. No. G595) was used to amplify different sized amplicons (500 bp, 1 kb, 2 kb, and 5 kb). Total reaction times are listed based on a 30-cycle program using the recommended reaction protocol for each enzyme.
Unsure which polymerase is right for you?
Unsure which polymerase is right for your application? Contact us for assistance or use the simple PCR Enzyme Selection Guide below to compare the characteristics of each polymerase.
| CHARACTERISTIC | MegaFi™ Fidelity | BlasTaq™ | BlasTaq™ HotStart |
|---|---|---|---|
| Proofreading | Yes | No | No |
| Fidelity (vs. Native Taq) | 1300X | 1X | 1X |
| Specificity | •••• | ••• | •••• |
| Extension Speed | 20-30 sec/kb | 15 sec/kb | 15 sec/kb |
| Target Length | 15-20 kb | 10-15 kb | 10-15 kb |
| Suitable for TA cloning | No | Yes | Yes |
| Special Feature | Fidelity and Sensitivity | Fast and Robust | High Specificity |
Request a Free Sample
See for yourself why your fellow PCR enthusiasts enjoy our product so much! Simply select the free product sample(s) you would like to receive and we will get in touch with you to coordinate the delivery to your lab. Please note that sample availability is limited and honored on a "first come first served" basis and shipping charges may apply.
Resources
Introduction to PCR
Polymerase Chain Reaction Knowledge Base
