Alkaline Phosphatase Conjugated Affinity Purified anti-Bovine IgG [H&L] [Goat]

CAT.NOUNITPRICE
SA001250 µg
$145.00

Specifications


SKUSA001
Unit quantity250 µg
Species ReactivityBovine
ImmunogenBovine IgG whole molecule
Applicationssuitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended DilutionsThis product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:600 to 1:3,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
Concentration0.6 mg/ml (by UV absorbance at 280 nm)
Storage ConditionStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Storage Buffer0.01% (w/v) Sodium Azide
Buffer0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Stabilizer10 mg/ml Polyethylene Glycol (PEG-8000)
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Bovine IgG and Bovine Serum.
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.
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