Alkaline Phosphatase Conjugated Affinity Purified anti-Bovine IgG [H&L] [Rabbit]
|Unit quantity||250 µg|
|Immunogen||Bovine IgG whole molecule|
|Applications||Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.|
|Recommended Dilutions||This product has been assayed against 1.0 µg of Bovine IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,500 to 1:6,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher.|
|Concentration||1.0 mg/ml (by UV absorbance at 280 nm)|
|Storage Condition||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.|
|Storage Buffer||0.01% (w/v) Sodium Azide|
|Buffer||0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Stabilizer||10 mg/ml Polyethylene Glycol (PEG-8000)|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine and Bovine Serum.|
|Caution||This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.|
There are no FAQs for this product yet!
There are no references for this product yet!