Alkaline Phosphatase Conjugated Affinity Purified anti-Bovine IgG [H&L] [Rabbit]

SA002250 µg


Unit quantity250 µg
Species ReactivityBovine
ImmunogenBovine IgG whole molecule
ApplicationsSuitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
Storage ConditionStore vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Storage Buffer0.01% (w/v) Sodium Azide
Buffer0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Stabilizer10 mg/ml Polyethylene Glycol (PEG-8000)
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Bovine and Bovine Serum.
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.

Supporting Protocol





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