Alkaline Phosphatase Conjugated Affinity Purified anti-Cat IgG [H&L] [Rabbit]
| CAT.NO | UNIT | PRICE |
|---|---|---|
| SA004 | 250 µg | $145.00 |
| SKU | SA004 |
|---|---|
| Unit quantity | 250 µg |
| Species Reactivity | Other |
| Immunogen | Cat IgG whole molecule |
| Applications | Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. |
| Recommended Dilutions | This product has been assayed against 1.0 µg of Cat IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:18,000 is suggested for this product. Optimal titers for other applications should be determined by the researcher. |
| Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
| Storage Condition | Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product. |
| Storage Buffer | 0.01% (w/v) Sodium Azide |
| Buffer | 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 |
| Stabilizer | 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free |
| Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Cat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Cat IgG and Cat Serum. |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
Supporting Protocol
MSDS
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