Alkaline Phosphatase Conjugated Affinity Purified anti-Swine IgG (H&L) [Goat]
|Unit quantity||250 µg|
|Immunogen||Swine IgG whole molecule|
|Applications||Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.|
|Recommended Dilutions||ELISA 1:2,000 - 1:10,000|
WESTERN BLOT 1:500 - 1:2,500
IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000
|Concentration||1.0 mg/ml (by UV absorbance at 280 nm)|
|Storage Condition||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.|
|Storage Buffer||0.01% (w/v) Sodium Azide|
|Buffer||0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Stabilizer||10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Swine IgG and Swine Serum.|
|Caution||This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.|
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