Anti-Beta-Actin Polyclonal Loading Control Antibody

CAT.NOUNITPRICE
G046100 μg
$225.00

Specifications


SKUG046
Unit quantity100 μg
Raised inRabbit
ClonalityMonoclonal
IsotypeIgG
Species ReactivityHuman
SpecificityRecognize β-actin from Human and Mouse. Other species not tested yet.
ImmunogenSyntheitic peptide at the N-terminus of Beta-Actin. The N-terminus of Beta-Actin is highly conserved with Gamma-Actin.
ApplicationsOptimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:500. IP: 5 mcg/mg lysate.
Recommended DilutionsOptimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:500. IP: 5 mcg/mg lysate.
FormulationLiquid
Concentration100ug/100ul
Storage ConditionThis product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage BufferPBS, pH 7.4 with 0.02% Sodium Azide
Guaranteeabm guarantees that all our Anti-Beta-Actin Polyclonal Loading Control Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
Documents


Supporting Protocol

    MSDS

      QC

        Other

          FAQs


          Which secondary antibody has been tested to be working for G046?
          All secondary antibodies that recognizes Rabbit IgG will work fine with cat#G046. For an applicable HRP conjugated secondary antibody, we recommend our cat#SH036 - Peroxidase Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat].
          References


          6
          • Chaiyakul, M et al. "Cytotoxicity of ORF3 proteins from a nonpathogenic and a pathogenic porcine circovirus" J Virol 84 (21):11440-11447 (2010). DOI: 10.1128/JVI.01030-10. Application: Western Blot.
          • Horakova, AH et al. "The macrosatellite DXZ4 mediates CTCF-dependent long-range intrachromosomal interactions on the human inactive X chromosome" Hum Mol Genet 21 (20):4367-4377 (2012). DOI: 22791747. Application: Western Blot.
          • Kim, BH et al. "Hairless plays a role in formation of inner root sheath via regulation of Dlx3 gene" J Biol Chem 287 (20):16681-16688 (2012). DOI: 10.1074/jbc.M111.320770. Application: Western Blot.
          • Sanz-Clemente, A et al. "Casein kinase 2 regulates the NR2 subunit composition of synaptic NMDA receptors" Neuron 67:984-96 (2010). PubMed: 20869595.
          • Kim, B.K. et al. "Hairless Up‐Regulates Tgf‐β2 Expression via Down‐Regulation of miR‐31 in the Skin of “Hairpoor”(HrHp) Mice" J. Cell. Physiol :2075–2085 (2015). DOI: 10.1002/jcp.24935.
          • Bong-Kyu, K.B et al. "miR‐330‐5p inhibits proliferation and migration of keratinocytes by targeting Pdia3 expression" FEBS Journal 24:1742-4658 (2015). DOI: 10.1111/febs.13523.