Anti-D Tag Antibody (Mouse Monoclonal)

CAT.NOUNITPRICE
G191100 μg
$155.00

Specifications


SKUG191
Unit quantity100 μg
Clone1.00E+06
Raised inMouse
ClonalityTag
IsotypeIgG2b with lambda light chain
Species ReactivityOther
SpecificityAnti-D tag Antibody recognises over-expressed proteins containing D epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
ImmunogenA synthetic peptide (DYKDDDDK) coupled to KLH.
Applications

Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western Blot at dilutions of 1:1000. Immunoprecipitation amd immunostaining at dilutions of 1:1000.

Recommended Dilutions
FormulationLiquid
Concentration100μg / 100μl
Storage Condition

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Storage BufferPBS, pH 7.4 with 0.05% sodium azide.
Guarantee

abm guarantees that all our Anti-D Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

Documents


Supporting Protocol

    MSDS

      QC

        Other

          FAQs


          What is the purification method for the tag antibodies?
          We use Protein A affinity beads for all tag antibody purification.
          References


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          • Neilson, KM et al. "Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate" Dev Biol 365 (2):353-375 (2012). DOI: 10.1016/j.ydbio.2012.03.004. Application: Western Blot.
          • Christova, T et al. "Lymphoid enhancer factor-1 mediates loading of Pax3 to a promoter harbouring lymphoid enhancer factor-1 binding sites resulting in enhancement of transcription" Int J Biochem & Cell Biol 42 (5):630-640 (2010). DOI: 10.1016/j.biocel.2009.12.006. Application: Western Blot.
          • Antony, S et al. "Characterization of NADPH oxidase 5 expression in human tumors and tumor cell lines with a novel mouse monoclonal antibody" Free Radic Biol Med. 65C:497-508 (2013). DOI: 10.1016/j.freeradbiomed.2013.07.005. Application: Western Blot.
          • Li, J et al. "Cell Cycle Regulator ING4 Is a Suppressor of Melanoma Angiogenesis That Is Regulated by the Metastasis Suppressor BRMS1" Cancer Res 70 (24):10445-10453 (2010). DOI: 10.1158/0008-5472.CAN-10-3040. Application: Immunoprecipitation.
          • Christova, T et al. "Sox4-mediated Dicer expression is critical for suppression of melanoma cell invasion" Oncogene 32 (17):2131-2139 (2013). DOI: 10.1038/onc.2012.239. Application: Western Blot.
          • Son, O et al. "In vitro and in vivo interaction of AtRma2 E3 ubiquitin ligase and auxin binding protein 1" Biochem Biophys Res Commun 393 (3):492-497 (2010). DOI: 10.1016/j.bbrc.2010.02.032. Application: Immunoblot.
          • Cheng, Y et al. "Prognostic significance of Fbw7 in human melanoma and its role in cell migration" J Invest Dermatol 133 (7):1794-802 (2013). DOI: 10.1038/jid.2013.58. Application: Western Blot.
          • Chen, G et al. "The tumor suppressor ING3 is degraded by SCFSkp2-mediated ubiquitin–proteasome system" Oncogene 29 (10):1498-1508 (2010). DOI: 10.1038/onc.2009.424. Application: Western Blot.
          • Li, J et al. "Prognostic significance of BRMS1 expression in human melanoma and its role in tumor angiogenesis" Oncogene 30 (8):896-906 (2011). DOI: 10.1038/onc.2010.470. Application: Western Blot.
          • Lu, J et al. "ING4 regulates JWA in angiogenesis and their prognostic value in melanoma patients" Br J Cancer 109 (11):2842-1852 (2013). DOI: 10.1038/bjc.2013.670. Application: Western Blot.
          • Lu, J et al. "JWA inhibits melanoma angiogenesis by suppressing ILK signaling and is an independent prognostic biomarker for melanoma" Carcinogenesis 34 (12):2778-2788 (2013). DOI: 10.1093/carcin/bgt318. Application: Western Blot.
          • Rotte, A et al. "Tumor suppressor Ing1b facilitates DNA repair and prevents oxidative stress induced cell death" Apoptosis 19:518-26 (2014). PubMed: 24242916.
          • Klein , SL et al. "Conserved structural domains in FoxD4L1, a neural forkhead box transcription factor, are required to repress or activate target genes" PLoS ONE 8:e61845 (2013). PubMed: 23610594. Application: Immunoprecipitation.
          • Jafarnejad, SM et al. "Role of Sox4 transcription factor in human cutaneous melanoma" Thesis : (2012).
          • Wang, Y et al. "Leucine zipper-like domain is required for tumor suppressor ING2-mediated nucleotide excision repair and apoptosis" FEBS Lett. 580:3787-93 (2006). PubMed: 16782091.
          • Lee, HS et al. "Fibroblast growth factor receptor-induced phosphorylation of ephrinB1 modulates its interaction with Dishevelled" Mol Biol Cell 20:124-33 (2009). PubMed: 19005214.
          • Prosser, DC et al. "FFAT rescues VAPA-mediated inhibition of ER-to-Golgi transport and VAPB-mediated ER aggregation" J Cell Sci 121:3052-61 (2008). PubMed: 18713837.
          • Jo, SH et al. "Txnip contributes to impaired glucose tolerance by upregulating the expression of genes involved in hepatic gluconeogenesis in mice" Diabetologia 2013:2723-32 (2013). PubMed: 24037087.
          • Tang, Y et al. "Prognostic significance of KAI1, CD82 in human melanoma and its role in cell migration and invasion through the regulation of ING4" Carcinogenesis 35:86-95 (2014). PubMed: 24130172.
          • Tran, D et al. "A mutation in VAPB that causes amyotrophic lateral sclerosis also causes a nuclear envelope defect" J Cell Sci 125:2831-6 (2012). PubMed: 22454507.
          • Rothenburg, S et al. "Characterization of a ranavirus inhibitor of the antiviral protein kinase PKR" BMC Microbiol. 11:56 (2011). PubMed: 21418572. Application: Western Blot.
          • Lee, HS et al. "EphrinB1 controls cell-cell junctions through the Par polarity complex" Nat. Cell Biol. 10:979-86 (2008). PubMed: 18604196.
          • Cho, HJ et al. "EphrinB1 Interacts with CNK1 and Promotes Cell Migration through JNK Activation" J. Biol. Chem. : (0). PubMed: 24825906.
          • Rothenburg, S et al. "Rapid evolution of protein kinase PKR alters sensitivity to viral inhibitors" Nat. Struct. Mol. Biol. 16:63-70 (2009). PubMed: 19043413.
          • Hwang, YS et al. "The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1" Genes Dev. 27:491-503 (2013). PubMed: 23475958.
          • Lee, JY et al. "Cell growth defect factor1, chaperone-like protein of POR1 plays a role in stabilization of light-dependent protochlorophyllide oxidoreductase in Nicotiana benthamiana and Arabidopsis" Plant Cell 25:3944-60 (2013). PubMed: 24151298.
          • Wong, RP et al. "Tumour suppressor ING1b maintains genomic stability upon replication stress" Nucleic Acids Res. 39:3632-42 (2011). PubMed: 21227930.
          • Jafarnejad, SM et al. "Sox4-mediated Dicer expression is critical for suppression of melanoma cell invasion" Oncogene 32:2131-9 (2013). PubMed: 22689055.
          • Chen, G et al. "The tumor suppressor ING3 is degraded by SCF(Skp2)-mediated ubiquitin-proteasome system" Oncogene 29:1498-508 (2010). PubMed: 19935701.
          • Rothenburg, S et al. "Double-stranded RNA-activated protein kinase PKR of fishes and amphibians: varying the number of double-stranded RNA binding domains and lineage-specific duplication" BMC Biol. 6:12 (2008). PubMed: 18312693. Application: Immunoblot.
          • Jafarnejad, SM et al. "Prognostic significance of Sox4 expression in human cutaneous melanoma and its role in cell migration and invasion" Am. J. Pathol. 177:2741-52 (2010). PubMed: 20952589. Application: Western Blot.
          • Ji, YJ et al. "EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1" Nat Commun 5:3516 (2014). PubMed: 24662724.
          • Cherry, AA et al. "Characterization of a homolog of DEAD-box RNA helicases in Toxoplasma gondii as a marker of cytoplasmic mRNP stress granules" Gene 1:34-44 (2014). PubMed: 24709106.
          • Ananvoranich, A et al. "Utilization of inherent miRNAs in functional analyses of Toxoplasma gondii genes" J Microbiol Methods :92-102 (2015). PubMed: 25479428.
          • Lee, E., Lee, T. A., Kim, J. H., Park, A., Ra, E. A., Kang, S., ... & Lee, S. "CNBP acts as a key transcriptional regulator of sustained expression of interleukin-6"  Nucleic acids research  45(6):3280-3296 (2017).