Anti-D Tag (Rabbit Polyclonal) Antibody

G188100 μg


Unit quantity100 μg
Raised inRabbit
Species ReactivityOther
SpecificityAnti-D tag Antibody recognises over-expressed proteins containing D epitope tag fused to either amino- or carboxy-termini of targeted proteins in transfected mammalian cells.
ImmunogenA synthetic peptide (DYKDDDDK) coupled to KLH.
ApplicationsOptimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western Blot at dilutions of 1:1000. Immunoprecipitation amd immunostaining at dilutions of 1:200.
Recommended Dilutions
Storage ConditionThis product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage BufferPBS, pH 7.4 with 0.05% sodium azide.
Guaranteeabm guarantees that all our Anti-D Tag (Rabbit Polyclonal) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

Supporting Protocol





          What is the purification method for the tag antibodies?
          We use Protein A affinity beads for all tag antibody purification.

          • Jo, SH et al. "Txnip contributes to impaired glucose tolerance by upregulating the expression of genes involved in hepatic gluconeogenesis in mice" Diabetologia 56 (12):2723-2732 (2013). DOI: 10.1007/s00125-013-3050-6. Application: Western Blot.
          • Lu, J. "JWA inhibits melanoma angiogenesis by suppressing ILK signaling and is an independent prognostic biomarker for melanoma" Carcinogenesis 34:2778-88 (2013). PubMed: 24064223.
          • Tran, D et al. "A mutation in VAPB that causes amyotrophic lateral sclerosis also causes a nuclear envelope defect" J Cell Sci 125:2831-6 (2012). PubMed: 22454507.
          • Rothenburg, S et al. "Double-stranded RNA-activated protein kinase PKR of fishes and amphibians: varying the number of double-stranded RNA binding domains and lineage-specific duplications" BMC Biol. 6:12 (2008). PubMed: 18312693. Application: Immunoblot.
          • Lee, H. K., Ismail, T., Kim, C., Kim, Y., Park, J. W., Kwon, O. S., ... & Lee, H. S. "Lysine demethylase 3a in craniofacial and neural development during Xenopus embryogenesis"  International journal of molecular medicine  43(2):1105-1113 (2019).
          • Lee, H., Ismail, T., Kim, C., Kim, Y., Park, J., Kwon, O., … Lee, H. "Lysine demethylase 3a in craniofacial and neural development during Xenopus embryogenesis" International Journal of Molecular Medicine. : (2018). DOI: 10.3892/ijmm.2018.4024.