Anti-F4/80 ( monocytes / Macrophages), murine Antibody
| Cat. No. | Y103412 |
| Name | Anti-F4/80 ( monocytes / Macrophages), murine Antibody |
| Clone | Monoclonal Antibody |
| Category | Monoclonal Antibodies |
| Gene Name | F4-80 ( monocytes - Macrophages), murine |
| Application | WB,IHC,IP |
| Application Info |
Suitable for use in Immunohistochemistry (Frozen 1:500, Paraffin 1:200), FACS, immunoprecipitation and Western Blot. |
| Raised In | Rat |
| Clonality | Monoclonal |
| Reactivity | Mouse |
| Description |
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins which shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells. F4/80 ( monocytes / Macrophages) clone number CI:A3-1. |
| Immunogen | Peritoneal mouse macrophages |
| Isotype | IgG2b |
| Concentration | 1mg/ml |
| Recommended Dilutions | Suitable for use in Immunohistochemistry (Frozen 1:500, Paraffin 1:200), FACS, immunoprecipitation and Western Blot. |
| Storage Buffer | PBS pH 7.2 with 0.1% Sodium Azide |
| Storage Condition |
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Species Reactivity | Mouse |
| Purity | Affinity purified |
| Guarantee |
abm guarantees that all our Anti-F4/80 ( monocytes / Macrophages), murine Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Y103412 |