Anti-GFP Tag Antibody (Goat Polyclonal)

CAT.NOUNITPRICE
G095100 μg
$295.00

Specifications


SKUG095
Unit quantity100 μg
CloneN/A
Raised inGoat
ClonalityTag
IsotypeIgG
Species ReactivityOther
SpecificityRecognize GFP and its variants.
ImmunogenThe anti-GFP immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
ApplicationsELISA 1:40,000; WESTERN BLOT 1:400 - 1:2,000; IMMUNOHISTOCHEMISTRY 1:200 - 1:1,000; IF MICROSCOPY 1:1,000; IP 1:200
Recommended Dilutions
FormulationLiquid
Concentration100ug/100ul
Storage ConditionThis product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage BufferPBS, pH 7.4 with 0.05% sodium azide.
Guaranteeabm guarantees that all our Anti-GFP Tag Antibody (Goat Polyclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse or Rat serum proteins.
Documents


Supporting Protocol

    MSDS

      QC

        Other

          FAQs


          What is the purification method for the tag antibodies?
          We use Protein A affinity beads for all tag antibody purification.
          References


          2
          • Sutton, LM et al. "Hip14l-deficient mice develop neuropathological and behavioural features of Huntington disease" Hum Mol Genet 22 (3):452-465 (2013). DOI: 10.1093/hmg/dds441. Application: Immunoprecipitation.
          • Luchniak, A et al. "Structure-function analysis of yeast" Methods Cell Biol 115:355-374 (2013). DOI: 10.1016/B978-0-12-407757-7.00022-0. Application: SDS-PAGE.