Anti-HA Tag Antibody (Mouse Monoclonal)
|Description||Hemagglutinin is an antigenic glycoprotein found on the surface of the influenza viruses, as well as many other bacteria and viruses. It is responsible for binding the virus to the cell that is being infected. The name hemagglutinin comes from the protein's ability to cause red blood cells (erythrocytes) to agglutinate in vitro.|
|Unit quantity||100 μg|
|Specificity||HA-tag monoclonal antibody. Recognizes HA-tagged tagged proteins overpressed in cells, including both amino- or carboxy-termini of targeted proteins in transfected mammalian cells.|
|Immunogen||A synthetic peptide from influenza hemagglutinin epitope (YPYDVPDYA) coupled to KLH.|
ICC/IHC/IF/IP 1:200 dilution. WB 1:1000 dilution.
|Recommended Dilutions||ICC/IHC/IF/IP 1:200 dilution. WB 1:1000 dilution.|
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
|Storage Buffer||PBS, pH 7.4 with 0.05% sodium azide.|
|Cellular Localization||Apical Cell Membrane|
abm guarantees that all our Anti-HA Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
|What does IS mean? (tested application)|
IS means immunostaining
|What dilution was used to achieve the WB and IF labeling pictured? What is the recommended dilution range for immunofluorescence?|
The recommended dilution range for immunofluorescence for G036 is to use at an assay dependent dilution. The dilution for the labeled picture is 1:500.
|What is the purification method for the tag antibodies?|
We use Protein A affinity beads for all tag antibody purification.
|What is the recommended dilution range for Western Blot?|
We recommend to start with 1:1000 but the researcher should then optimized if needed for their experiments.
|What is the size of the HA tag?|
The immunogen sequence for the HA tag is YPYDVPDYA, which is about 1.1 kDa.
|What is the dilution recommended for immunoprecipitation?|
This product is provided with a recommended starting dilution for WB only. It is not yet tested in other applications such as IP, therefore optimal dilutions/concentrations should be determined by the end user.
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