Anti-HA Tag Antibody (Rabbit Polyclonal)
|Unit quantity||100 μg|
|Specificity||HA-Tag polyclonal antibody detects HA-tagged proteins overexpressed in cells. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.|
|Immunogen||The antiserum was produced by immunizing rabbit with synthesized peptide containing the influenza hemagglutinin epitope (Y-P-Y-D-V-P-D-Y-A).|
|Recommended Dilutions||WB: 1:500~1:1000|
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
|Storage Buffer||phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
abm guarantees that all our Anti-HA Tag Antibody (Rabbit Polyclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
|Purity||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.|
|What is the purification method for the tag antibodies?|
We use Protein A affinity beads for all tag antibody purification.
- Nam, G. et al. "Interaction of CD99 with its paralog CD99L2 positively regulates CD99L2 trafficking to cell surfaces." J Immunol 191(11):5730-42 (2013). DOI: 10.4049/jimmunol.1203062. PubMed: 24133166 . Application: Immuno Blotting.
- Segal, I., Nachmias, D., Arbely, E., & Elia, N. "A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag" : (2019). DOI: 10.1101/708545.