Anti-HA Tag Antibody (Rabbit Polyclonal)

CAT.NOUNITPRICE
G166100 μg
$180.00

Specifications


SKUG166
Unit quantity100 μg
ClonePolyclonal
Raised inRabbit
ClonalityTag
IsotypeIgG
Species ReactivityOther
SpecificityHA-Tag polyclonal antibody detects HA-tagged proteins overexpressed in cells. The antibody recognizes the HA-tag fused to either the amino- or carboxy-terminus of targeted proteins in transfected mammalian cells.
ImmunogenThe antiserum was produced by immunizing rabbit with synthesized peptide containing the influenza hemagglutinin epitope (Y-P-Y-D-V-P-D-Y-A).
Applications

WB: 1:500~1:1000

Recommended DilutionsWB: 1:500~1:1000
FormulationLiquid
Concentration100ug/100ul
Storage Condition

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Storage Bufferphosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Guarantee

abm guarantees that all our Anti-HA Tag Antibody (Rabbit Polyclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Documents


Supporting Protocol

    MSDS

      QC

        Other

          FAQs


          What is the purification method for the tag antibodies?
          We use Protein A affinity beads for all tag antibody purification.
          References


          2
          • Nam, G. et al. "Interaction of CD99 with its paralog CD99L2 positively regulates CD99L2 trafficking to cell surfaces." J Immunol 191(11):5730-42 (2013). DOI: 10.4049/jimmunol.1203062. PubMed: 24133166 . Application: Immuno Blotting.
          • Segal, I., Nachmias, D., Arbely, E., & Elia, N. "A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag" : (2019). DOI: 10.1101/708545.