Anti-Myc Tag Antibody (Rabbit Polyclonal)
|Description||Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve a universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.|
|Unit quantity||100 μg|
|Specificity||This polyclonal anti-Myc-tag antibody detects overexpressed proteins containing the Myc epitope tag. The antibody recognizes the Myc-tag (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.|
|Immunogen||Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L|
|Applications||ELISA 1:1,000 ; WESTERN BLOT 1:500 - 1:1,000 ; IMMUNOHISTOCHEMISTRY User Optimized ; OTHER APPLICATIONS User Optimized|
|Recommended Dilutions||ELISA 1:1,000 ; |
WESTERN BLOT 1:500 - 1:1,000 ;
IMMUNOHISTOCHEMISTRY User Optimized ;
OTHER APPLICATIONS User Optimized
|Storage Condition||This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.|
|Storage Buffer||PBS, pH 7.4 with 0.05% sodium azide.|
|Guarantee||abm guarantees that all our Anti-Myc Tag Antibody (Rabbit Polyclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.|
|What is the purification method for the tag antibodies?|
We use Protein A affinity beads for all tag antibody purification.
- Kim, JH et al. "The Arabidopsis RING E3 ubiquitin ligase AtAIRP3, LOG2 participates in positive regulation of high-salt and drought stress responses" Plant Physiol 162:1733-49 (2013). PubMed: 23696092.