Autism Spectrum Disorder Control Fibroblastic Cell Line (sc148)
|T6364||5x105 cells / 1.0 ml|
Autism Spectrum Disorder Control Fibroblastic Cell Line (sc148) was isolated from a neurologically normal volunteer. Autism spectrum disorders (ASD) are poorly understood and a new panel of Idiopathic Autism Spectrum Disorder Fibroblastic Cell Lines are now available at abm for research needs. These will provide the ability to evaluate and compare data from a number of different cell lines which will facilitate greater insight into the cause of the ASDs and will be extremely useful for uncovering new therapeutic and diagnostic targets.
|Species||Human (H. sapiens)|
|Species description||Homo sapiens|
|Donor Age||56 years old|
For Research Use Only
|Unit quantity||5x105 cells / 1.0 ml|
|Cell Type||Drug Discovery Cell Lines|
Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add fetal bovine serum (TM999) to a final concentration of 10%, MEM non-essential amino acids solution (Gibco) to a final concentration of 1%, GlutaMAX (Gibco) to a final concentration of 1% and penicillin-streptomycin solution (G255) to a final concentration of 1%,
1. Pre-warm the complete growth media specified by the cell line datasheet in a 37°C water bath. Pre-warm the Trypsin-EDTA (TM050) to room temperature.
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Depositor||Innovation Lab, LLC|
Brick, D. J., Nethercott, H. E., Montesano, S., Banuelos, M. G., Stover, A. E., Schutte, S. S., … Schwartz, P. H. (2014). The Autism Spectrum Disorders Stem Cell Resource at Children’s Hospital of Orange County: Implications for Disease Modeling and Drug Discovery. STEM CELLS Translational Medicine, 3(11), 1275–1286. doi: 10.5966/sctm.2014-0073.
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